离子色谱-脉冲安培检测白醋和豆腐水中单糖和大豆低聚糖  

Determination of Monosaccharides and Soybean Oligosaccharides in White Vinegar and Tofu Water by Ion Chromatography and Pulsed Amperometric Detection

作　　者：李仁勇[1,2] 梁立娜[1,2] 牟世芬[1] 蔡亚岐[1] 

机构地区：[1]中国科学院生态环境研究中心,环境化学与生态毒理学国家重点实验室,北京100085 [2]戴安中国有限公司应用研究中心,北京100085

出　　处：《分析化学》2009年第5期725-728,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20621703);国家863重大课题(No.2007AA06A407)资助项目

摘　　要：建立了测定白醋和豆腐水中常见的的3种单糖(半乳糖、葡萄糖、果糖),以及蔗糖、蜜二糖、棉子糖和水苏糖等大豆低聚糖的方法。以NaOH淋洗液梯度淋洗,7种糖在CarboPac PA10高效阴离子交换柱(HPAEC)上可以在30 min内完成分离。使用脉冲安培检测器(PAD)进行测定,7种糖的检出限(进样25μL,S/N=3)分别为3、2、3、6、4、7和6μg/L,且均具有较宽的线性范围(0.02～20 mg/L)。白醋和豆腐水样品测定的相对标准偏差在0.15%～3.16%之间,7种糖的加标回收率在92%～104%之间;本方法检测糖简便快捷、分离效果好、无需衍生、灵敏度高,适用于大豆及大豆制品中的常见糖组分的分析。A chromatographic method was developed for the determination of galactose, glucose, fructose, sucrose, melibiose, raffinose and stachyose in white vinegar and tofu water. These seven saccharides can be separated well in 30 min by CarboPac PA10 high performance anion exchange column（HPAEC） with sodium hydroxide gradient elution. The detection limits of galactose, glucose, fructose, sucrose, melibiose, raffinose and stachyose（25 μL injection, S/N =3） using Pulsed Amperometric Detection（PAD） are 3, 2, 3, 6, 4, 7 and 6 μg/L, respectively. Moreover, all analytes have wide linearity range（0.02 -20 mg/L） , good relative standard deviations（0.2% - 3.2% ） and satisfying recoveries （92% - 104% ）. This method is quite suitable for the determination of common saccharides in soybeans and soybean products for its simple and good separa- ting performance as well as high sensitivity. No derivation is needed in the process of determination.

关 键 词：离子色谱法 脉冲安培检测 白醋 豆腐水 大豆低聚糖 

分 类 号：TS207.3[轻工技术与工程—食品科学]