阿胶生产工艺中胶原蛋白降解过程研究  

Investigation on degradation process of collagens during manufacturing procedure of E′jiao

作　　者：张贵锋[1] 刘涛[1] 王前[1] 罗坚[1] 解茹[1] 秦玉锋[2] 尤金花[2] 田守生[2] 苏志国[1] 

机构地区：[1]中国科学院过程工程研究所生化工程国家重点实验室,北京100190 [2]山东东阿阿胶股份有限公司,山东东阿252201

出　　处：《中国中药杂志》2009年第10期1211-1215,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(20706052);国家高技术研究发展计划863项目(2007AA021604)

摘　　要：目的:研究阿胶生产工艺中胶原蛋白的降解过程以及关键单元操作。方法:在阿胶生产过程中取样并分析其氨基酸组成和相对分子质量范围,利用高效液相色谱质谱技术分析其多肽组成。结果:阿胶生产工艺中原料胶中甘氨酸含量提高了16.8%,表明原料胶中胶原蛋白含量逐渐升高;凝胶过滤色谱分析结果表明原料胶相对分子质量逐渐降低,胶原蛋白的降解过程主要集中在常压浓缩阶段;质谱分析结果表明原料胶内除胶原蛋白外还存在细胞外基质蛋白和部分细胞骨架蛋白的降解物,在常压浓缩过程中以泡沫形式被去除的物质主要为非胶原类蛋白的降解物。结论:阿胶生产工艺中胶原蛋白的降解过程主要集中在常压浓缩阶段,常压浓缩过程是影响阿胶产品组成的关键单元操作。Objective： To investigate the degradation process of collagens and identify the key unit operation during manufacturing process of E＇jiao. Method： Samples in different unit operations were withdrawn, and their amino acid compositions and the molecular weight ranges were determined. The peptide composition was analyzed by high-performance liquid chromatography/mass spectrometry. Result： The content of sample during atmospheric condensation unit increased by 16. 8% compared to the thermal extraction unit. Gel filtration chromatographic analysis indicated that the degradation process of collagen primarily occurred during the atmospheric condensation unit. The peptides in samples mainly resulted from the degradation of collagens and cytoskeleton proteins such as tubulin, actinin, and so on. The relative abundance of degraded collagens increased with the decrease of no-collagen proteins. Conclusion： Collagen degradation mainly occurred during the atmospheric condensation unit, which was the key process affecting the composition of E-jiao.

关 键 词：阿胶 生产工艺 胶原蛋白 降解 高效液相色谱质谱联用 

分 类 号：TQ461[化学工程—制药化工]