正交旋转组合设计优化豆豉纤溶酶Subtilisin FS33的培养条件  

Medium improvement by orthogonal rotary combination design for Subtilisin FS33 production by Bacillus subtilis DC_(33)

作　　者：王成涛[1,2] 籍保平[2] 孙宝国[1] 曹雁平[1] 李涛[2] 

机构地区：[1]北京工商大学化学与环境工程学院,北京100037 [2]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《中国调味品》2009年第6期50-53,共4页China Condiment

基　　金：国家重点基础研究发展计划资助项目“973”计划(2007CB707802)

摘　　要：从中国传统豆豉中分离筛选出一株高产纤溶酶菌株Bacillus subtilis DC33,并研究营养和环境因素条件对DC33产酶的影响。在单因素水平试验基础上,选择对DC33产纤溶酶影响较大的聚蛋白胨、半乳糖、硫酸镁、硫酸锂和吐温80五因素,通过正交旋转组合设计得到DC33产酶活力与营养因素水平的回归方程,极值分析得DC33优化发酵培养基(g/L):聚蛋白胨22,半乳糖1.56,硫酸镁0.5,硫酸锂0.01,K2HPO42.0,NaH2PO41.0,CaCO32.0,吐温80 0.33 mL。在此条件下,DC33产纤溶酶达到780 U/mL,较优化前提高86.7%。Subtilisin FS33 is a new fibrinolytic enzyme, which cleaves directly cross-linked fibrin in vitro. Medium improvement for the production of FS33 by B. subtilis DC33 was investigated using an orthogonal rotary combination design. Results revealed that tryptone, galactose, magnesium sulphate, lithium sulphate and Tween 80 had a significant effect on enzyme production. The improved medium consisted of 22.0 g/L tryptone, 1.56 g/L galactose, 0. 5 g/L magnesium sulphate, 0.01 g/L lithium sulphate, 2. 0 g/L K2HPO4, 1.0 g/L KH2PO4, 2.0 g/L CaCO3 and 0.33 mL/L Tween 80. Subtilisin FS33 production reached 780 U/mL at 72 h in the optimized condition and was about 186.7 % greater than with the control medium.

关 键 词：豆豉纤溶酶 BACILLUS SUBTILIS DC33 正交旋转组合设计 发酵培养基优化 

分 类 号：TS201.25[轻工技术与工程—食品科学]