Localization and Dynamic Change of Saponin in Vegetative Organs of Polygala tenuifolia  

作　　者：Hong-Mei Teng Min-Feng Fang Xia Cai Zheng-Hai Hu 

机构地区：[1]Key Laboratory of Resource Biology and Biotechnology in West China (Northwest University), Ministry of Education, Xi'an 710069, China [2]Department of Life Sciences, Yuncheng Coflege, Yuncheng 044000, China

出　　处：《Journal of Integrative Plant Biology》2009年第6期529-536,共8页植物学报（英文版）

基　　金：Funded Projects of Independent Innovation of NorthwestUniversity Postgraduates (07YZZ25);the Shaanxi Provincial NaturalScience Foundation (SJ08-ZT02)

摘　　要：Anatomical, histochemical and phytochemical methods were used to investigate the structure, localization and dynamic changes of total saponin and senegenin of vegetative organs in Polygala tenuifolia Willd. Histochemical localization results showed that saponin accumulated mainly in parenchyma cells of vegetative organs. The phytochemical results also showed that the saponin accumulated in the vegetative organs of P. tenuifolia, with higher content in roots and lower content in the aerial parts that included stems and leaves. The saponin content and dry weight of the vegetative organs of P. tenuifolia had dynamic variance at the developmental stages and all reached the highest level in the post-fruit period. Hence, the roots and aerial parts should be gathered in August to make full use of the plant. As the root is the main medicinal organ of P. tenuifolia, the content of total saponin and senegenin of different aged and different parts of the root were determined. The content of total saponin and senegenin exhibited a sustained decreasing trend with increasing root age; therefore, the annual roots had high quality. The content of total saponin and senegenin in different parts of the root showed obvious variation. The content in the ＂skin areas＂ was much higher than that of xylem. The results offer a theoretical basis for determining the appropriate harvesting stage and a reasonable harvest of P. tenuifolia.Anatomical, histochemical and phytochemical methods were used to investigate the structure, localization and dynamic changes of total saponin and senegenin of vegetative organs in Polygala tenuifolia Willd. Histochemical localization results showed that saponin accumulated mainly in parenchyma cells of vegetative organs. The phytochemical results also showed that the saponin accumulated in the vegetative organs of P. tenuifolia, with higher content in roots and lower content in the aerial parts that included stems and leaves. The saponin content and dry weight of the vegetative organs of P. tenuifolia had dynamic variance at the developmental stages and all reached the highest level in the post-fruit period. Hence, the roots and aerial parts should be gathered in August to make full use of the plant. As the root is the main medicinal organ of P. tenuifolia, the content of total saponin and senegenin of different aged and different parts of the root were determined. The content of total saponin and senegenin exhibited a sustained decreasing trend with increasing root age; therefore, the annual roots had high quality. The content of total saponin and senegenin in different parts of the root showed obvious variation. The content in the ＂skin areas＂ was much higher than that of xylem. The results offer a theoretical basis for determining the appropriate harvesting stage and a reasonable harvest of P. tenuifolia.

关 键 词：dynamic change LOCALIZATION Polygala tenuifolia SAPONIN SENEGENIN STRUCTURE vegetative organs. 

分 类 号：Q593.9[生物学—生物化学] Q944.53