植物乳杆菌Lp-2分批发酵工艺的研究  

Study on Batch Fermentation Technology of Lactobacillus plantarum Lp-2

作　　者：张广敏[1,2] 王炜[1,3] 包慧芳[1,3] 张晓琳[1,2] 崔卫东[1,3] 詹发强[1,3] 林瑞峰 

机构地区：[1]新疆农业科学院微生物应用研究所,新疆乌鲁木齐830091 [2]石河子大学农业生物技术重点实验室,新疆石河子832003 [3]新疆特殊环境微生物工程技术研究中心,新疆乌鲁木齐830091

出　　处：《食品与发酵工业》2009年第3期36-40,共5页Food and Fermentation Industries

基　　金：国家973计划"秸秆资源生态高值化关键过程的基础研究"(2004CB719704);国家农业科技成果转化资金项目"青贮饲料微生物添加剂技术成果转化"(2006GB2G400336);新疆自治区科研院所专项资金项目"青贮饲料发酵过程中微生物动态变化规律研究"(20080018)

摘　　要：研究和发酵条件对植物乳杆菌Lp-2高密度增殖的影响。经过对缓冲盐法和化学中和法对比,确定了植物乳杆菌高密度增殖发酵最佳方法为化学中和法并对其进行了详尽地研究。最后,进行了培养工艺的放大。试验表明,在32℃、100r/min的培养条件下在3t发酵罐培养9.6h,所得菌体的最大产量为9.3×109cfu/mL。This paper studied the effects of fermentation condition on the high cell density culture of Lactobacillus plantarum Lp-2. Then the method of the high cell density culture was determined between phosphate buffer and flowing-alkaline. The method of flowing-alkaline was explored thoroughly and in details. At last, the large-scale culture technology and the scale up of Lp-2 culture process were studied. The maximum yields of Lactobacillus plantarura Lp-2 can be obtained at the speed of 100 r/min, temperature at 32℃ for 9.6h in a 3 t fermentor. The maximum yields of Lactobacillus plantarum Lp-2 was 9.3 × 10^9cfu/mL. The result showed that our culture optimization was effective, feasible, and could be scaled to high volume manufacturing.

关 键 词：缓冲盐 流加碱中和 高浓度细胞悬浮液 

分 类 号：TQ921[轻工技术与工程—发酵工程]