Fe_3O_4/海藻酸盐微胶囊固定化细胞生长特性  

The Growth Property of Cell Immobilized in Fe_3O_4/Alginate-based Microcapsules

作　　者：温惠云[1] 齐智涛[1] 薛伟明[1] 薛美辰[1] 孙维娜[1] 沈艳佐[1] 

机构地区：[1]西北大学化工学院,陕西西安710069

出　　处：《食品与发酵工业》2009年第4期51-55,共5页Food and Fermentation Industries

基　　金：国家自然科学基金资助项目(No.50373046);陕西省教育厅专项科研计划资助项目(No.07JK386);西北大学研究生创新基金资助项目(No.07YZZ23;08YZZ49);陕西省"13115"科技创新工程重大科技专项(2008ZBKG-58)

摘　　要：采用脉冲电场液滴制备工艺,以海藻酸钠、壳聚糖为载体材料,纳米Fe3O4粉末为磁性材料,地衣芽孢杆菌为细胞模型,制备具有超顺磁性的微尺度载细胞微胶囊。以地衣芽孢杆菌的生长量为考察指标,考察不同磁含量、磁场引力、粒径和初始菌浓等因素的影响。实验结果表明,磁性材料Fe3O4的引入对地衣芽孢杆菌的生长没有影响。制备磁性固定化地衣芽孢杆菌微胶囊的最适条件为:Fe3O4含量0.003g/mL,初始接种密度为1.5倍菌浓(6.25×106个/mL),微囊的平均粒径为350μm,磁场环境的引入对地衣芽孢杆菌的生长无明显影响。论证了磁性壳聚糖/海藻酸钙微胶囊固定化细胞工艺进行在线分离,连续化操作的可行性。Taking chitosan and sodium alginate as the carrier,nano-Fe3O4 as the magnetic material,B, lichenifor mis as the cell model, magenetic microcapsule which has super-magnetism was prepared by the Impulsive Electrostatic Preparation process. Choosing the growth of B. licheniformis as the detecting target , the optimum condition to prepare magnetic immobilized B. licheniformis were as follows ： Fe3O4 concentration 0. 003 g/mL ; the initial inoculation density was 1.5 times（6.25 × 10^6 cells/mL） ; the average diameter of magnetic microcapsules were 350 μm. It also showed that the introduction Of magnetic field had no significant effect . The study proved the possibility to separate on-line of the magnetic material and immobilized cell Fe3O4 chitosan/calcium alginate microcapsules and kept continuous operation.

关 键 词：壳聚糖 海藻酸钠 FE3O4 固定化细胞 微胶囊 

分 类 号：Q813[生物学—生物工程]