微生物发酵产生的番茄红素的分离纯化  

Separation and Purification of Lycopene Obtained from Blakeslea trispora

作　　者：苗振华[1] 郑珩[1] 劳兴珍[1] 王聪[1] 顾觉奋[1] 强晓妍[1] 

机构地区：[1]中国药科大学生命科学与技术学院,南京210009

出　　处：《氨基酸和生物资源》2009年第2期4-8,共5页Amino Acids & Biotic Resources

基　　金：国家大学生创新实验计划

摘　　要：研究了三孢布拉霉(Blakeslea trispora)发酵产生的番茄红素的分离纯化方法。与植物源的番茄红素相比,真菌发酵产物中含有更多的油脂成分,且提取物中含有的一些同分异构体与产物性质类似而不易分离。实验采用溶剂抽提、大孔吸附树脂吸附法纯化以及结晶的方法可获得番茄红素晶体,纯度达70.9%,收率为64.6%。产品经重结晶后可获得纯度90%以上的番茄红素,经红外、核磁、质谱等证实与植物来源的番茄红素相一致。Separation and purification of lycopene produced by Blakeslea trispora was studied. Compared with the method of tomato extraction, lycopene - producing fungi contained more lipids. It was hard to separate lycopene from other isomerides in the extract. The hyphostroma was extracted by petroleum ether and then purified by macroporous adsorption resin X - 5 and then crystallized under - 20 ℃. The purity of crystal was 70.9% and yield was 64.6%. After further recrystallisation the structure was confirmed by IR, 1H - NMR and MS.

关 键 词：大孔吸附树脂 真菌 发酵法 番茄红素 分离纯化 

分 类 号：TQ028.15[化学工程]