丹酚酸B酶解产物的分离  

Purification of the enzymatic products of Salvianolic acid B

作　　者：于翀[1] 王莹[1] 鱼红闪[1] 金凤燮[1] 

机构地区：[1]大连工业大学生物与食品工程学院,辽宁大连116034

出　　处：《大连工业大学学报》2009年第3期161-164,共4页Journal of Dalian Polytechnic University

基　　金：国家自然科学基金资助项目(30470055);辽宁省教育厅创新团队项目(2007T006)

摘　　要：利用Sepabeads SP207大孔吸附树脂分离提纯丹酚酸B酶解产物。经Sepabeads SP207大孔吸附树脂的水洗和乙醇溶液梯度洗脱,分离得到丹酚酸B酶解产物的两种单体,为产物Ⅰ和产物Ⅱ。用薄层层析法和高效液相色谱法检测,得到产物Ⅰ为丹参素,得率为17.6%,纯度为99%;产物Ⅱ得率为41.0%,纯度为91%。其结构、性质及药理作用有待于进一步研究。When Salvianolic acid B hydrolysate was applied on macroporous resin Sepaheads SP207 and eluted with water and ethanol. Two monomers of Salvianolic acid B hydrolysate were obtained. The hydrolysate Ⅰ was identified as danshensu by TLC and HPLC. The yield of the danshensu is 17.6%, the purity of which is 99%. The yield of hydrolysate Ⅱis 41.0%, the purity of which is 91%. Its structure needs further investigation.

关 键 词：丹酚酸B 酶解产物 大孔吸附树脂 分离 

分 类 号：TS972.161[轻工技术与工程] R284.2[医药卫生—中药学]