马铃薯中马来酰肼的高效液相色谱法测定  

Determination of Maleic Hydrazide Residue in Potatoes Using High Performance Liquid Chromatography

作　　者：裴明黎[1] 蒋振辉[1,2] 王秋霜[3] 应铁进[1] 

机构地区：[1]浙江大学生物系统工程与食品科学学院,浙江杭州310029 [2]江苏大学食品与生物工程学院,江苏镇江212013 [3]广东省农业科学院茶叶研究所,广东广州510640

出　　处：《分析测试学报》2009年第5期611-613,616,共4页Journal of Instrumental Analysis

摘　　要：建立了马铃薯中马来酰肼的高效液相色谱(HPLC)检测方法。马铃薯样品经酸化甲醇提取,高速离心沉淀分离后,以甲醇-去离子水(体积比1:9,pH2.7)为流动相,采用ZORBAXSBC18柱(250mm×4.6mm×5μm)分离,在UV205nm检测,外标法定量。结果表明,马来酰肼提取液的线性范围为0.2～16.0mg/L,回收率为82%～94%,RSD均小于2%,马铃薯中马来酰肼的定量下限为1.0mg/kg。A method for the determination of maleic hydrazide in potatoes was established by high performance liquid chromatography. Potato samples were separated with ZORBAX SB Cls column （250 mm ×4. 6 mm ×5 μm） using methanol -water（10 ： 90, pH 2.7） as mobile phase, after ex- tracted by acidific methanol and preseperated by centrifugal precipitation. It was quantified by exter- nal standards method and determined at UV 205 nm. Results showed that the linear range for the ma- leic hydrazide extraction was 0. 2 - 16. 0 mg/L and the detection limit was 1.0 mg/kg. Recoveries were between 82% - 94% with RSDs less than 2%. This method could be used to determine the maleic hydrazide in potatoes.

关 键 词：高效液相色谱 马铃薯 马来酰肼 

分 类 号：O657.72[理学—分析化学] Q949.777.7[理学—化学]