分光光度法测定胭脂红酸的研究  

Determination of carminic acid by spectrophotometry

作　　者：张益峰[1] 朱鑫雁[1] 王素芳[1] 汪财生[1] 

机构地区：[1]浙江万里学院生物与环境学院,浙江宁波315100

出　　处：《中国酿造》2009年第6期152-154,共3页China Brewing

基　　金：宁波市青年基金(2005A620013);浙江省新苗计划(2007G60G2210020)

摘　　要：建立了分光光度法检测胭脂红酸的方法。结果表明,pH4.0浓度为1.0mol/L的磷酸钠缓冲液较适宜作胭脂红酸的溶解剂,胭脂红酸在1.25μg/mL～160μg/mL的浓度范围内的吸光度值线性相关系数达0.9997,方法回收率为104%～110%,平均相对标准偏差为0.78%～2.13%。A method for determination of carminic acid by spectrophotometry was established. The results showed that the 1.0mol/L sodium phosphate buffer at pH 4.0 was appropriate to dissolve carminic acid. When the concentration of carminic acid ranged between 1.25 and 160μg/mL, the linearly dependent coefficient of absorbanee reached 0.9997.the recovery rate was 104%- 110%, and the average relative standard deviation was 0.78% - 2.13%.

关 键 词：分光光度法 胭脂红酸 检测 

分 类 号：O657.32[理学—分析化学]