维生素E对铁和乙醇诱导的大鼠睾丸脂质过氧化反应的保护作用  

Protection of Vitamine E against Testes Lipid Peroxidation Induced by Iron and Ethanol

作　　者：张雪生[1] 柳启沛[1] 哈捷 

机构地区：[1]上海医科大学公共卫生学院

出　　处：《卫生研究》1998年第3期184-186,共3页Journal of Hygiene Research

基　　金：国家教委研究生科研经费资助

摘　　要：观察了急性铁和乙醇中毒对大鼠睾丸组织氧化和抗氧化系统的影响以及补充维生素Ｅ（ＶＥ）的保护作用。结果显示：急性铁中毒可以引起铁在睾丸组织明显蓄积，而乙醇可加剧此作用。与对照组比较，急性铁中毒组、急性铁和乙醇中毒组，睾丸组织中铁含量分别增加６．８和９．１倍。随着睾丸组织中铁含量的增加，组织中脂质过氧化产物（２－ｔｈｉｏｂａｒｂｉｔｕｒｉｃａｃｉｄ－ｒｅｃｔｉｖｅｓｕｂｓｔａｎｃｅｓ，ＴＢＡＲＳ）含量明显增加，脂溶性抗氧化维生素ＶＥ含量明显减少。急性铁和乙醇中毒未见对抗氧化酶有明显影响。结果提示：铁在乙醇所致的睾丸组织脂质过氧化反应中起十分重要的作用，与长期饮酒导致男（雄）性生育能力下降甚至不育可能有关系。he influence of acute iron and ethanol load on lipid peroxidation and antioxidative defense systems in rat testes and the modification after supplemented with Vitamin E was investigated.Acute iron and ethanol load was achieved by i.p.injection of iron dextran(500mg/kg)and ethanol(50mmol/kg).After 18h,a significant increase in testis total iron content was induced.Compared with control,total testis iron content was 6.8 fold higher in iron treated rats and 9.1 fold in iron and ethanol treated rats.As the content of iron increasing,the endogenous lipid peroxidation evaluated as 2 thiobarbituric acid rective substances(TBARS)increased apparently,and the content of lipid soluble antioxidants αtocopherol decreased.The supplement of an antioxidant,αtocopherol,protected against lipid peroxidation.Iron and ethanol treatment did not affect the activity of superoxide dismutase,catalase and glutathione peroxidase.The results indicate that acute iron load causes iron accumulation in rat testes and ethanol increases the same accumulation.Iron played pivotal paracrine roles on ethanolinduced injure rat testis.The supplement of Vitamin E can protect against this damage.

关 键 词：铁 乙醇 睾丸 脂质过氧化 维生素E 

分 类 号：R155.3[医药卫生—营养与食品卫生学] R977.25[医药卫生—公共卫生与预防医学]