胸腺素α原反义寡核苷酸对胃癌细胞生长的影响  

Effect of Prothymosin alpha Antisense Oligodeoxynucleotide on Human Gastric Cancer in Vitro.

作　　者：王梅[1] 潘吉勇[2] 安利佳[3] 

机构地区：[1]大连医科大学病理学与法医学教研室,辽宁大连116044 [2]大连市第三人民医院普通外科,辽宁大连116033 [3]大连理工大学生物科学与生物技术系,辽宁大连116024

出　　处：《现代生物医学进展》2009年第11期2051-2054,2015,共5页Progress in Modern Biomedicine

摘　　要：目的:探讨反义胸腺素α原(Prothymosin alpha,ProTα)基因转染对胃癌细胞生长的影响,为以ProTα为靶向的胃癌基因治疗开辟新的途径。方法:人工合成ProTα反义寡核苷酸(ProTα-AS-ODN),以阳离子聚合物转染法转染体外培养的人胃癌细胞BGC-823,以正义寡核苷酸和未转染组为对照,应用RT-PCR及免疫细胞化学(immunocytochemistry,ICC)法分析ProTα基因及其蛋白的表达,MTT法分析细胞生长抑制作用,Hoechst33258检测细胞凋亡。结果:RT-PCR、ICC显示ProTα-AS-ODN转染组ProTα mRNA及其蛋白的表达显著减少,MTT检测表明ProTα-AS-ODN转染组活细胞数较其它各组均低(P<0.05),Hoechst33258则显示其凋亡细胞数较其它各组明显增高(P<0.01)。结论:ProTα-AS-ODN成功转染人胃癌细胞BGC-823,封闭了ProTαmRNA的翻译,使已转染的细胞ProTα蛋白的表达减少,且可抑制细胞的增殖并诱导细胞发生凋亡。Objective： To study the effects of Prothymosin alpha （ProT） antisense oligodexynucleotide （ProTα-AS-ODN） on human gastric cancer cells and to deploy a possible theapeutic approach of ProTα-targeted in human gastric adenocarcinomas. Methods： Antisense phosphorothioate oligodeoxynucletides targeted against ProTα mRNA were transiently transfected into BGC-823 cells to down-express ProTα. The altered expression of ProTα mRNA and protein were examined by RT-PCR and immunocytochemistry （ICC）. Then, MTT assay and Hoechst 33258 staining were used to detect cytotoxicity and apoptosis. Results： The down-expressed ProTα may induce a complete inhibition of cell proliferation to apoptosis in a dose-dependent manner in BGC-823 cells. Conclusion： The efforts aiming at cutting down ProTα expression may prove to be an attractive alternative therapy against gastric cancer.

关 键 词：胃癌 反义寡核苷酸 胸腺素Α原 

分 类 号：R730.54[医药卫生—肿瘤]