PCR-DHPLC技术快速检测食品中英诺克李斯特氏菌  

Identification of Listeria innocua in food by PCR-DHPLC

作　　者：曹际娟[1] 王耀[1,2] 王顺芝 李天顺[1] 李建民 赤列加措 

机构地区：[1]辽宁出入境检验检疫局,辽宁大连116001 [2]沈阳农业大学植保学院,辽宁沈阳110161 [3]西藏出入境检验检疫局,西藏拉萨850002

出　　处：《辽宁师范大学学报（自然科学版）》2009年第2期235-238,共4页Journal of Liaoning Normal University:Natural Science Edition

基　　金：国家"十一五"科技支撑计划课题(2006BAK02A13)

摘　　要：应用PCR结合变性高效液相色谱(DHPLC)技术,建立食品中英诺克李斯特氏菌的快速检测方法.根据英诺克李斯特氏菌的特有基因设计特异性引物,PCR扩增的产物经DHPLC技术进行快速检测,并以41株参考菌株做特异性试验.试验结果表明该方法具有很好的特异性,经PCR-DHPLC可检测食品中英诺克李斯特氏菌.该方法可以快速、准确检测英诺克李斯特氏菌,是食品微生物快速检测的新技术.To identify Listeria innocua in food, a PCR-Denaturing High-Performance Liquid Chromatography （DHPLC） assay was performed in this study. Specific primers for specific gene of Listeria innocua were selected to conduct the PCR-DHPLC assays. The specific testing was performed with 41 strains. The good specificity of this method was found in the study. Also, it can detect and identify Listeria innocua quickly and accurately by a PCR-DHPLC. The DHPLC could be a new method for detecting pathogens in food.

关 键 词：PCR—DHPLC 食品 英诺克李斯特氏菌 

分 类 号：TS207.4[轻工技术与工程—食品科学]