酒精发酵培养基优化过程中两种试验设计方法的比较研究  

Comparison of Two Design Methods for the Optimization of Culture Mediums for Alcohol Fermentation

作　　者：解玉红[1] 郑岩[1] 丁宏亮[1] 闵笛[1] 徐立君[1] 冯炘[1] 

机构地区：[1]天津理工大学环境科学与安全工程学院,天津300384

出　　处：《酿酒科技》2009年第6期20-23,27,共5页Liquor-Making Science & Technology

基　　金：2009年天津市自然科学基金重点项目:"降解秸秆类固废微生物资源选育与其生态化综合利用研究"(09JCZDJC26200);天津市科委自然科学基金项目(043611111)

摘　　要：应用均匀设计和响应曲面设计两种设计方法对酒精发酵培养的配方进行了优化。同时,运用两种软件Minitab15和SAS9.0对所得数据进行了回归分析和结果计算。结果表明,两种设计方法所得到的培养基的配方基本一致。但是,均匀设计方法运用SAS9.0进行回归分析所用的实验次数比响应曲面法运用的试验次数较少,并且最终的乙醇产量高于响应曲面设计,所以本实验最终选择均匀设计方法,运用SAS9.0进行数据分析。Two design methods including response surface design （RSD） and uniform design （UD） were adopted for the optimization of culture medium for alcohol fermentation. Meanwhile, two softwares including Minitabl5 （for RSD） and SAS9.0 （for UD） were used for regression analysis and data calculation. The experimental results showed that there was almost no difference in culture medium formulas designed by the two methods, however, the testing times by UD were less than that by RSD and alcohol yield by UD was higher than that by RSD. Therefore, UD （SAS9.0 for data analysis） was finally adopted in practice.

关 键 词：酒精发酵 培养基 优化 设计方法 

分 类 号：TS262.2[轻工技术与工程—发酵工程] TS261.4[轻工技术与工程—食品科学与工程]