紫苏挥发油化学成分GC/MS分析及抑菌评价研究  

GC/MS ANALYSIS OF VOLATILE OIL OF Perrilla frutenscens L. AND EVALUATION OF ITS INHIBITING ABILITY TO BACTERIA

作　　者：林硕[1] 邵平[1] 马新[1] 孙培龙[1] 

机构地区：[1]浙江工业大学生物与环境学院,浙江杭州310014

出　　处：《核农学报》2009年第3期477-481,共5页Journal of Nuclear Agricultural Sciences

摘　　要：采用水蒸汽蒸馏法分别提取紫苏叶和花中挥发油,运用毛细管气相色谱-质谱联用法结合计算机检索对其挥发油进行化学成分分析,采用面积归一法测定了挥发油中各种成分的相对质量分数,利用抑菌贴片法、MIC法和光电浊度法分别对枯草芽孢杆菌和大肠杆菌进行了抑菌性研究。结果显示,从紫苏叶和花水蒸汽蒸馏所得挥发油中分别检测出12种和10种化合物,其中叶中主要成分为紫苏醛(75.88%)、柠檬烯(3.82%)、石竹烯(9.87%)和法呢烯(3.21%);花中主要成分为紫苏醛(49.23%)、石竹烯(18.53%)、法呢烯(8.99%)、芳樟醇(11.3%)和姜黄二酮(4.55%)。紫苏精油对两种细菌均有很强的抑制作用,但对枯草芽孢杆菌的抑制能力要明显强于对大肠杆菌的抑制能力。The constituents of essential oils extracted with steam distillation from leaves and flowers of Perrilla frutenscens L. were analyzed by GC/MS technique and their relative concentration was acquired with the methods of area normalization. The inhibiting ability to bacteria of the oils was also studied with a gram-positive bacteria Bacillus subtilis and a gram-negtive bacteria Escherichia coli as the objects by disc diffusion method, MIC and scattered light turbidity method. The results showed that 12 and 10 compounds were identified respectively from the leaves and flowers, and the main components from the leaves were perillaldehyde （75.88%）, limonene （3.82%）, caiyophilene （9.87%）, and farnesene （3.21%） ; the main components from the flowers were perillaldehyde （ 49.23% ）, caiyophilene （ 18.53% ）, famesene （ 8.99% ）, linalool （11.3%）, curdione （4.55%）. Moreover, the essential oils showed strong inhibiting activity against both bacteria, but having higher ability against Bacillus subtilis than that against Escherichia coli.

关 键 词：紫苏挥发油 水蒸汽蒸馏 GC/MS分析 抑菌能力 

分 类 号：S636.9[农业科学—蔬菜学]