复合菌固态发酵高蛋白饲料添加剂条件的优化  

Composite Bacteria of Solid State Fermentation Conditions of High Protein Feed Additive Optimization.

作　　者：卢洪栋[1] 彭爱娟[1] 徐志旭[1] 

机构地区：[1]郑州牧业工程高等专科学校质量检测与管理系,河南郑州450011

出　　处：《郑州牧业工程高等专科学校学报》2009年第2期3-6,共4页Journal of Zhengzhou College of Animal Husbandry Engineering

摘　　要：以黑曲霉菌、产朊假丝酵母和枯草芽孢杆菌为出发菌株，通过对固体发酵工艺条件和培养基优化的研究，获得了一条优化后的复合菌固态发酵高蛋白饲料添加剂生产工艺。研究表明以黑曲霉菌、产朊假丝酵母和枯草芽孢杆菌接人固体比例为4：3：3．固体接种量50％，发酵温度30℃～32℃，含水量50％，发酵初始pH4．6左右，发酵时间50h为发酵条件，以豆饼粉85％，（NH4）：SO40．8％。KH：PO40．2％。麸皮15％．硫酸铜和硫酸锰分别加入0．8‰为固体培养基，所产酸性蛋白酶活力最高为15426u／g，而获得最大酵母菌菌数为35亿．小肽含量为12．86％。粗蛋白含量为52．32％的高蛋白饲料添加剂。To black Aspergillus and Candida yeast and Bacillus subtilis as the starting strain, through the fermentation process to solid conditions and optimization of the medium, it was an optimization to a composite of solid state fermentation of high protein feed additive production process. Research shows that a black Aspergillus and Candida yeast and Bacillus subtilis access solid ratio of 4..3：3, solid vaccination of 50 %, the fermentation temperature 30℃-32℃, water content of 50%. the initial fermentation pH 4. 6 About 50h fermentation time for fermentation conditions,Soybean powder to 85%, （ NH4 ）：SO4 0. 8%, KH： PO4 0.2%, wheat bran 15%, copper sulfate and manganese sulfate respectively 0.8 % to to solid medium, it produced by a maximum of protease activity 15426 u / g, while it got the maximum number of yeast to 3.5 billion, small peptide content of 12. 86 %, the crude protein content of 52. 32 % of the high protein feed additive.

关 键 词：复合菌 酸性蛋白酶 小肽 固态发酵 

分 类 号：S816.7[农业科学—饲料科学]