肺癌候选抑瘤基因HLCDG1片段的验证及全长序列的克隆  

Testing and rapid amplification of 3'/5' cDNA end of a candidate tumor suppressor gene HLCDG1 in human lung cancer

作　　者：邹飞雁[1] 谢海龙[2] 余艳辉[3] 欧阳咏梅[3] 贺智敏[3] 陈主初[3] 

机构地区：[1]暨南大学生科院生殖免疫研究所,广东广州510632 [2]南华大学肿瘤研究所细胞生物研究室,湖南衡阳421001 [3]中南大学湘雅医学院肿瘤研究所细胞生物室,湖南长沙410078

出　　处：《中国病理生理杂志》2009年第6期1081-1088,共8页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30471954);中国博士后科学基金资助项目(No.2003034467);暨南大学自然科学基金资助项目

摘　　要：目的:对肺癌候选抑瘤基因HLCDG1片段(已知序列)进行验证,并克隆其全长cDNA序列。方法:采用RT-PCR和末端快速扩增技术(RACE),对正常人肺组织中HLCDG1基因片段进行验证和克隆其全长cDNA序列,登录基因数据库进行比对分析。结果:从正常人肺组织中钓取到HLCDG1基因未知的3’端和5’端序列分别为1 304 bp和1 548 bp。HLCDG1基因与人酪蛋白激酶Ⅰα(CSNK1A1)基因2号转录变体相似性达99%,score=5 544,E=0。结论:HLCDG1基因为已知的CSNK1A1基因的2号转录变体,可能参与肺癌发生发展。AIM ： To obtain HLCDG1 full cDNA sequence based on part of known cDNA sequence from human lung cancer. METHODS： In human lung tissue the rapid amplification of cDNA ends （RACE） was used to clone 3＇ - terminal and 5＇ - terminal of HLCDG1 cDNA, and identified by sequencing, RT - PCR and BLAST. RESULTS ： 3＇ - terminal and 5＇ - terminal unknown cDNA of HLCDG1 amplified were 1 304 bp and I 548 bp, respectively. The HLCDG1 full - length sequence had a sequence homology with casein kinase 1, alpha 1, transcript variant 2 （ CSNKIA1 - TV2）, and the identity was 99%, the score was 5 544 bits, and the expect was 0. CONCLUSION： HLCDG1 gene is the CSNK1A1 gene, which will be helpful for further assessment its biological function （s） in carcinogenesis of lung cancer.

关 键 词：基因 HLCDG1 基因 CSNK1A1 DNA末端快速扩增法 肺肿瘤 

分 类 号：R739.62[医药卫生—肿瘤]