从白蚁中分离筛选纤维素分解菌及其产酶性质  

Screening and Crude Enzyme Property of a Cellulose-Decomposing Strain

作　　者：何刚强[1,2] 堵国成[2] 刘立明[2] 霍光华[1] 

机构地区：[1]江西农业大学生物科学与工程学院,江西南昌330045 [2]江南大学工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《食品与生物技术学报》2009年第3期352-355,共4页Journal of Food Science and Biotechnology

基　　金：国家973计划项目(2007CB714306)

摘　　要：以羧甲基纤维素钠(CMC-Na)为惟一碳源对白蚁悬液进行富集培养,并从中分离菌株32株,通过纤维素刚果红初筛平板获得透明圈较大的8株菌,在此基础上进行摇瓶复筛,得到1株酶活较高的菌株(B3)。同时对其酶促反应温度、稳定性及其酶作用底物进行了测定。结果表明:CMCase的最适酶促反应温度为50℃,且在该温度下有较强的稳定性,保温30 min酶活基本保持不变,60 min酶活损失约14%,菌株B3所产纤维素酶对玉米秸秆纤维素粉有较强的水解能力,同时对滤纸和脱脂棉也有一定的降解能力。Thirty-two strains were isolated from termite suspension with CMC-Na as the sole carbon source. We got eight colonies surrounded by some big transparent zone in cellulose-congo red agar medium. A strain （B3） of cellulose-decomposing strain was isolated by shake flask for the second screening. The optimal reacting temperature of cellulase secreted by strain B3 was 50 ℃. The specific enzyme activity was similar when temperature was kept at 50 ℃ for 30 min. However, for the case of 60 min, the enzyme activity lost about 14%. Under the same conditions, the straw enzyme activity was very high, and relative high FTA and absorbent cotton enzyme activity was observed.

关 键 词：白蚁 纤维素分解菌 酶活 酶学性质 

分 类 号：Q96[生物学—昆虫学]