抑瘤素-M(OSM)在原核系统中的表达  

Expression of oncostatin M gene in E.coli

作　　者：曹勇[1] 李淑琴[1] 张兆山[1] 文立民[1] 

机构地区：[1]军事医学科学院生物工程研究所

出　　处：《军事医学科学院院刊》1998年第2期88-91,共4页Bulletin of the Academy of Military Medical Sciences

摘　　要：目的和方法：抑瘤素－Ｍ是一种具有多种生物活性的细胞因子，采用ＰＣＲ的方法在ＯＳＭ全长的ｃＤＮＡ基础上截去编码Ｃ端的３１个氨基酸的核苷酸序列，将其分别克隆至ｐＢＶ２２０和ＴｈｉｏＦｕｓｉｏｎＴＭ表达系统中进行表达，并对其包涵体进行初步的变性、复性和活性测定。结果和结论：证实了截去Ｃ端３１个氨基酸之后，ＯＳＭ仍保持其抑制活性。构建重组质粒ｐＢＶ２２０－ＯＳＭ、ｐＴＦ－ＯＳＭ。Objective and Methods: Oncostatin M (OSM) is an important cytokine which has diverse biological activities. After the cleavage of the sequence which codes the C terminal 31 amino acids by PCR, the cDNA of mature OSM was cloned into pBV220 and ThioFusion system TM . Results and Conclusion: The result of activity analysis showed that the OSM without C terminal 31 amino acids still had full inhibitor activity. The level of expression of fusion protein was about 15% of total cell proteins by SDS PAGE analysis.

关 键 词：抑瘤素-M PBV220 PCR扩增 原核系统 表达 

分 类 号：Q78[生物学—分子生物学]