丙烯和1-MCP处理对采后柿果实ACS和ACO基因表达的影响  

Effects of Propylene and 1-Methylcyclopropene on Expressions of ACC Synthase and ACC Oxidase Genes in Persimmon Fruits

作　　者：刘乐[1] 饶景萍[1] 常晓晓[1] 弋顺超[1] 

机构地区：[1]西北农林科技大学园艺学院/农业部西北园艺种质资源与遗传改良重点开放实验室,陕西杨凌712100

出　　处：《中国农业科学》2009年第6期2092-2097,共6页Scientia Agricultura Sinica

基　　金：国家自然科学基金项目(30170662;30771756)

摘　　要：【目的】研究丙烯、1-甲基环丙烯(1-MCP)处理对采后柿果实中与乙烯合成相关的ACS、ACO基因特异表达的影响。【方法】以中国涩柿的优良品种‘富平尖柿’为试材,于初熟期采收后将果实分别用丙烯、1-MCP处理,在常温下贮藏,定期检测乙烯释放速率及分组织提取RNA进行Northern分析。【结果】丙烯处理促进ACS、ACO基因表达进而促进内源乙烯合成;1-MCP处理抑制ACS、ACO基因的表达从而抑制内源乙烯合成。不过,二者对ACS、ACO基因表达的作用效果在不同组织中差异较大:1-MCP处理对ACS、ACO基因表达的抑制作用在果皮中最明显,其次是果肉、果心部位,果蒂着生部位处最低;丙烯对ACS、ACO基因表达的促进作用表现为果肉、果心、果皮、果蒂着生部位递增。另外,ACS、ACO基因家族各成员在不同组织的表达也不同,DKACS3基因只在果蒂着生部位表达在其它组织中没有表达,DKACS2基因在丙烯处理果中的4个组织中均有表达。【结论】丙烯处理促进ACS、ACO基因表达,1-MCP处理抑制ACS、ACO基因的表达;ACS、ACO基因在不同组织中的表达差异较大;ACS、ACO基因家族各成员在不同组织中的表达也不同。[Objective] The effects of postharvest treatment with propylene and 1-MCP on ethylene production and expressions ofACS andACO genes in Fupingjianshi persimmon （Diospyros kaki L.） fruit were investigated. [Method] Fruits were treated with propylene and 1-MCP, than stored at 20℃ ethylene released rate of the treated fruits were measured at regular intervals and RNA was extracted for Northern blotting analysis. [Result] Treatment with propylene accelerated the expression of ACS and ACO genes and enhanced the ethylene biosynthesis, while treatment with 1-MCP inhibited the expression of two genes and their ethylene biosynthesis. Furthermore, different effects on expressions caused by treatments with propylene and 1-MCP existed in various fruit tissues. The inhibitory effect on ACS and ACO genes by 1-MCP was the strongest in pulp and core tissues, followed by pericarp and the area near fruit stalk while the enhanced effect on ACS and ACO genes by propylene increased from pulp, core, and pericarp to the area near fruit stalk. Expression of each member of ACS and ACO families in various tissues were also completely different, ACS3 gene just expressed in the area near fruit stalk and didn＇t express in other tissues, but ACS2 gene expressed in four tissues by treatment with propylene. [ Conclusion ] Treatment with propylene accelerated the expression of ACS and ACO genes, while treatment with 1-MCP inhibited the expression of ACS and ACO genes. Different effects on expressions caused by treatments with propylene and I-MCP existed in various fruit tissues. Expression of each member of ACS and ACO families in various tissues were also completely different.

关 键 词：柿 丙烯 1-MCP ACC合成酶 ACC氧化酶 

分 类 号：S665.2[农业科学—果树学]