UV-B诱导的酵母凋亡现象及调节机制的作用  

UV-B Irradiation Regulates Apoptosis in Yeast

作　　者：赵华[1] 伍丹[1] 

机构地区：[1]天津科技大学生物工程学院教育部工业微生物重点实验室,天津300457

出　　处：《微生物学通报》2009年第6期826-830,共5页Microbiology China

摘　　要：研究UV-B诱导的酵母凋亡现象及调节机制的作用。通过高密度细胞培养,UV-B能够抑制酵母细胞生长和诱导细胞凋亡。然而,将UV-B已照射96h活酵母细胞重新进行UV-B照射时发现,培养12d照射过细胞的存活率仍有10%(P<0.05),而未照射细胞已经基本死亡。同时,经0.01mol/L和0.1mol/LH2O2处理,UV-B照射24h活细胞的存活率分别是对照的3.0倍和5.2倍;而经30min和60min55°C热处理,UV-B照射24h活细胞的存活率分别是对照的3.5倍和9.0倍。Mechanisms of UV-B-induced apoptotic regulation in yeast Saccharomyces cerevisiae were studied. The results showed that UV-B irradiation indeed inhibited the growth of yeast cells as well as induced extensive apoptosis during 96 h experiment period. However, survival of 96 h irradiated cells remained 10% while most control cells finally dead after re-growth under UV-B irradiation for 12 d. And by exposed to 0.01 mol/L or 0.1 mol/L H2O2 for 30 min, survival rate of 24 h irradiated cells were 3.0-fold or 3.2-fold than control, respectively. By to heat shock for 30 min or 60 min, survival rate of 24 h irradiated cells were 3.5-fold or 9.0-fold than control, respectively.

关 键 词：UV-B 酵母细胞 细胞凋亡 凋亡调节机制 

分 类 号：Q93[生物学—微生物学]