重组大肠杆菌高密度发酵生产类人胶原蛋白Ⅱ条件优化  

Optimization of Recombinant E. coli High-density Fermentation for Expressing Human-like CollagenⅡ

作　　者：常海燕[1,2,3] 范代娣[2,3] 骆艳娥[2,3] 马晓轩[2,3] 米钰[2,3] 朱晨辉[2,3] 迟雷[2,3] 

机构地区：[1]西北大学生命科学学院,陕西西安710069 [2]西北大学陕西省可降解生物医用材料重点实验室,陕西西安710069 [3]陕西省生物材料与发酵工程技术研究中心,陕西西安710069

出　　处：《微生物学通报》2009年第6期870-874,共5页Microbiology China

基　　金：国家863计划资助项目(No2006AA02Z246);国家自然科学基金项目(No20476085;20776619);陕西省教育厅科研计划项目(No07JK417)

摘　　要：通过考察pH、培养温度、溶解氧浓度和诱导时机对细胞生长和类人胶原蛋白Ⅱ表达的影响,确定这些因素的最佳控制范围,优化发酵条件。结果表明:控制初始pH为6.5,诱导后pH为6.8,培养温度34°C,DO20%及在对数生长后期进行诱导,有利于细胞生长和外源基因的表达,最终细胞密度为88.4g/L,类人胶原蛋白Ⅱ产量达到14.2g/L。To study and optimize the fermentation parameters for expressing human-like collagenⅡduring E. coli high-density fermentation. The effects of pH, temperature, dissolved oxygen and induction instant on the cell growth and human-like collagenⅡproduction were investigated to optimize the fermentation conditions. The results demonstrated that the following conditions were beneficial for cell growth and foreign gene expression, controlling pH in phase induction at 6.8 and initial pH at 6.5, maintaining fermentation temperature and dissolved oxygen concentration was controlled at 34℃ and 20% respectively, and implementing induction at the later logarithmic growth phase. Under the optimized condition, the cell density and human-like collagenⅡyield could reach 88.4 g/L and 14.2 g/L, respectively.

关 键 词：类人胶原蛋白Ⅱ 高密度发酵 发酵条件 诱导时机 

分 类 号：Q78[生物学—分子生物学]