腈水合酶生产菌工业发酵技术研究  

Studying on Ferment Technology of the Nitrile Hydratase Producing Strain

作　　者：周云霞 胡毅 高俊 李红梅 刘晶 

机构地区：[1]大庆炼化公司,黑龙江大庆163411

出　　处：《精细与专用化学品》2009年第12期23-25,28,共4页Fine and Specialty Chemicals

摘　　要：丙烯酰胺是一种重要的有机化工原料．以它为中间体合成的产品有上百种，其聚合物或共聚物可用作化学灌浆物料、土壤改良剂、絮凝剂、纤维改性剂和涂料等。目前丙烯胺酰的生产工艺有丙烯腈硫酸化学水解法、丙烯腈以还原铜为金属催化剂的化学水合法和丙烯腈以腈水合酶为生物催化剂的微生物法。微生物法比化学法更具有优势．丙烯腈水合反应的条件温和．在常温常压下进行．反应后丙烯腈的转化率高达99．99％，反应液中的丙烯腈的含量可控制在50ppm以下．不需要去除和回收残余丙烯腈。产品的丙烯腈含量指标就完全合格，省去了残留丙烯腈去除和回收工艺流程．减少了设备投资、能耗和环境污染。且生物催化剂具有成本低、催化效率和选择性高的特点，因此微生物法获得的丙烯酰胺产品中杂质含量低．产品活性高．特别适合生产高分子量的聚合物。微生物法丙烯酰胺生产技术以其众多的优点成为了第三代丙烯酰胺生产技术。并在实际生产中得到了广泛应用．目前单线生产能力在2万t/a以上．The fermentation technology for production of strain via nitrile hydratase process was studied based on the factors such as medium compositions, operating technique for sterilizing, germ quality and pH value control influencing greatly on fermentation process was studied. The optimum medium compositions, the reasonable operating technique for sterilization, and the controlling method of germ quality and control of pH value were confirmed. In the practical applications, notable results were achieved, i.e. the period of fermentation was shortened by 20h or more, the nitrile hydratase activity was increased by more than 40%.

关 键 词：腈水合酶 生产菌 发酵技术 丙烯腈含量 生物催化剂 丙烯酰胺 微生物法 工业 

分 类 号：TQ925[轻工技术与工程—发酵工程] TQ922.9