多功能细菌复合系NSC-7的菌种组成多样性  

Composition Diversity of the Multifunctional Bacterium Community NSC-7

作　　者：刘长莉[1,2] 王小芬[2] 牛俊玲[3] 吕育财[2] 郭鹏[2] 沈海龙[4] 崔宗均[2] 

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040 [2]中国农业大学农学与生物技术学院,生物质工程中心,北京100094 [3]郑州航空工业管理学院资源与环境研究所,郑州450015 [4]东北林业大学林学院,哈尔滨150040

出　　处：《环境科学》2009年第7期2112-2117,共6页Environmental Science

基　　金："十一五"国家科技支撑计划重大项目(2006BAD07A01);东北林业大学青年基金项目(07035)

摘　　要：NSC-7是一组具有降解纤维素和林丹双重功能的细菌复合系.为系统了解复合系的菌种组成,在有氧条件下,利用传统的平板画线法分离到11株单菌,将11株单菌按体积比1∶1重新组合并不具备分解纤维素的能力,利用单层和双层平板滤纸法检测NSC-7的分解能力,发现只有双层平板上的滤纸变黄且降解,说明复合系内纤维素降解的关键菌是厌氧或微好氧菌.利用现代分子生物学技术对NSC-7构建克隆文库,获得了195个16SrDNA片断,经DGGE筛选获得25个代表克隆,其序列数据库比对结果中有60%的近缘种为已知菌,分别归属于Clostridium、Petrobacter、Bacteria、Paenibacillus、Proteobacterium 5个属,其余40%的近缘种为难培养菌株.The NSC-7 microbial community could decompose cellulose and lindan with high efficiency. In order to determine the bacterial composition of the community, 11 isolate strains were detected by plate isolation, while a community reset by the 11 isolate strains lost the capacity of degrading cellulose. The capacity of degrading of the filter paper in double deck plate and monolayer plate were determined, only the filter paper in double deck plate were degraded, that means the main or key microbe are anaerobic. The denaturing gradient gel eleetrophoresis （DGGE） and construction of 16S rDNA clone library were used to identify the composition diversity of NSC-7 community. 195 clones and 25 strains were detected in clone library, and about 60 % closest relative among them was known the detailed information which were belonged to Clostridium, Petrobacter, Bacteria, Paenibacillus, Proteobacterium. Furthermore, there were 40 % closest relative belonged to uncultured bacterium clone.

关 键 词：细菌复合系 木质纤维素 克隆文库 菌种组成多样性 

分 类 号：X172[环境科学与工程—环境科学]