一株产木聚糖酶的耐冷皮壳正青霉菌鉴定与酶谱分析  

Identification of A Novel Cold-adaptive Xylanolytic Eupenicillium crustaceum and Zymogram Analysis of Xylanase

作　　者：朱会芳[1] 江正强[1] 赵兰英 郝姗姗[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]葫芦岛市海洋与渔业环境监测中心站,葫芦岛125000

出　　处：《应用与环境生物学报》2009年第3期376-379,共4页Chinese Journal of Applied and Environmental Biology

基　　金：高等学校博士学科点专项科研基金资助课题;高等学校全国优秀博士学位论文作者专项资金(No.200555)~~

摘　　要：从青海冻土中分离得到1株产木聚糖酶的青霉菌QH11,通过菌落特征观察、显微特征观察和18SrDNA同源性序列分析,鉴定该菌为皮壳正青霉,其最适生长温度为20℃.该菌以玉米芯水不溶木聚糖为碳源,于20℃摇瓶培养,d4木聚糖酶达到高峰,酶活为28.13U/mL,比酶活为9.73U/mg,粗酶液的最适温度为47.5℃.SDS-PAGE和酶谱分析表明,该菌可产生3种木聚糖酶,其亚基相对分子质量(M)分别为20.5×103、23.0×103和35.0×103.A Novel cold-adaptive xylanolytic strain QHll was isolated from a tundra in Qinghai, China. The QHll was identified as a strain ofEupenicillium crustaceum by its morphological features and 18S rDNA sequence analysis, and it grew optimally at 20℃. This strain produced a maximal xylanase activity of 28.13 U/mL with the specific activity of 9.73 U/mg after 4 d cultivation in the medium containing insoluble corncob xylan as carbon source at 20 ℃. The optimal temperature for the xylanase activity was 47.5 ℃. SDS-PAGE and zymogram analysis of culture supernatant revealed that QHll secreted three xylanases with the subunit molecular masses of 20.5×10^3, 23.0×10^3 and 35.0×10^3, respectively.

关 键 词：木聚糖酶 耐冷菌 皮壳正青霉 酶谱分析 

分 类 号：Q93[生物学—微生物学]