中药阿胶的质量控制方法研究  

Investigation on Quality Control of E-Jiao by Mass Spectrometry

作　　者：张贵锋[1] 刘涛[1] 王前[1] 罗坚[1] 秦玉峰[2] 尤金花[2] 田守生[2] 余蓉[3] 苏志国[1] 

机构地区：[1]中国科学院过程工程研究所生化工程国家重点实验室,北京100190 [2]山东东阿阿胶股份有限公司,山东东阿县252201 [3]四川大学华西药学院靶向药物及释药系统教育部重点实验室,四川成都610041

出　　处：《药物生物技术》2009年第3期250-254,共5页Pharmaceutical Biotechnology

基　　金：自然科学基金项目(20706052);国家863项目(20007AA021604)

摘　　要：使用胰蛋白酶将阿胶、牛皮胶和猪皮胶进行了酶切处理,利用高效液相色谱质谱联用技术比较了3种胶酶解产物中多肽组成的差异。结果表明阿胶、牛皮胶和猪皮胶的酶解产物中存在有氨基酸序列差异的特征多肽,通过检测样品胶中特征多肽的序列可鉴别样品胶的种类。实验使用该方法检测了16种不同产地的阿胶样品,其中2种样品中检测出猪皮胶的特征多肽,1种胶中检测出牛皮胶的特征多肽。实验对阿胶预处理、酶解方法以及质谱分析过程中影响多肽识别的因素进行了研究。结果表明通过特征多肽追溯样品胶的动物来源是一种阿胶质量控制的有效方法。E-jiao （Donkey-skin gelatin）, bovine-skin gelatin and porcine-skin gelatin were used as standards and digested by trypsin, and the digestive mixtures were analyzed by high performance liquid chromatography/mass spectrometry （HPLC/MS）. Maker peptides specific for E-jiao, bovine- and porcineskin gelatin were identified respectively in three digested standards. Sixteen actual samples were analyzed by same method. It was found that two samples contained marker peptides specific for porcine gelatin, and one sample contained marker peptides specific for bovine gelatin. Thus, the animal source of gelatin sample can be traced according to the sequence of marker peptides in the digestive mixture. The results indicated that enzymatic digestion and detection of marker peptides by HPLC/MS was an effective method for quality control of E-jiao.

关 键 词：阿胶 质量控制 酶解 特征 高效液相色谱质谱 

分 类 号：R284.1[医药卫生—中药学]