甜叶悬钩子植物组织培养褐化现象控制研究  

Study on the Browning Control in Tissue Culture of Rubus suavissimus S.Lee

作　　者：郭治友[1] 钱绍方[1] 罗应[1] 

机构地区：[1]黔南民族师范学院生命科学系民族生物资源研究所,贵州都匀558000

出　　处：《安徽农业科学》2009年第19期8848-8850,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]寻求甜叶悬钩子组织培养中的最佳抗褐化剂。[方法]以甜叶悬钩子的茎和叶为外植体,以MS为基本培养基,加入不同浓度的抗褐化剂(20%Na2S2O3、Vc和AC),研究不同抗褐化剂对甜叶悬钩子组织培养的影响。[结果]AC和Vc都能对不同外植体起到抗褐化作用,在加入20%Na2S2O3的培养基上,外植体的褐化程度与Na2S2O3的浓度成正比,培养基中20%Na2S2O3的添加量达20m l时,外植体褐化最为迅速;在加入Vc的培养基上,随着Vc添加量的增加,培养基变成液体或半固体培养基,接入的外植体容易死亡;在加入AC的培养基上,AC的添加量为5 g/L时,外植体的褐化率可控制在5%以下。抗褐化控制处理后,可以在诱导愈伤组织的培养基上培养出大量的愈伤组织。[结论]AC是较为理想的抗褐化剂,用量为5 g/L。[ Objective ] The purpose was to seek for optimum anti-browning agent in the tissue culture of Rubus suavissimus S. Lee. [ Method ] With stems and leaves of R. suavissimus as explants, MS basic medium was used as the media by adding different concn, of anti-browning agent （20% Na2S2O3, Vc and AC） so as to research the effect of different anti-browning agent on tissue culture of R. suavissimus.. [ Result ] AC and Vc had anti-browning effect on different explants. On the medium with 20% Na2S2O3 , the browning extent of explants was proportional to the conch, of Na2S2O3. When the addition of 20% Na2S2O3 amounted to 20 ml, the browning of the explant was the fastest. On the medium with Vc, with the increasing addition of Vc, the medium got into a liquid or semi-selid medium and the explants died easily. On the medium with AC, when the addition of AC was 5 g/L, the browning rate of the explants could be controlled below 5%. A large number of callus could be induced on the culture medium after the anti-browning control treatment. [ Conclusion] AC was the best anti-browning agent and dosage was 5 g/L.

关 键 词：甜叶悬钩子 植物组织培养 褐化控制 

分 类 号：S571.1[农业科学—茶叶生产加工]