2种提取猪肝基因组DNA方法的比较  

Comparison of Two Methods of Genomic DNA Extraction from Pork Liver

作　　者：周春丽[1] 刘华[1] 李玉萍[1] 

机构地区：[1]江西科技师范学院生命科学学院,江西南昌330013

出　　处：《安徽农业科学》2009年第18期8355-8356,共2页Journal of Anhui Agricultural Sciences

基　　金：江西科技师范学院院级项目(2006);江西省教育厅科技项目(赣教技字[2007]285号);江西科技师范学院博士科研启动基金(2005)

摘　　要：[目的]对2种猪肝基因组DNA提取方法,进行比较研究。[方法]分别采用试剂盒法和常规的氯仿/异戊醇抽提法,提取新鲜猪肝基因组DNA,并对其进行综合分析。[结果]试剂盒方法得到的DNA浓度较低,其OD260nm/OD280nm比值达到2.05,虽然蛋白质除的较干净,但是含RNA较高;而氯仿/异戊醇抽提法得到的DNA浓度高,其OD260nm/OD280nm比值1.88,纯度较高,且成本低。[结论]氯仿/异戊醇法对猪肝基因组DNA提取效果优于试剂盒法。[Objective] The research aimed to compare the two extraction methods of genomic DNA from pork liver.[Method] kit method and chloroform/isoamyl alcohol extraction method were adopted to extract genomic DNA from fresh pork liver.[Result] The results showed that the DNA extraction kit could obtain high purity genomic DNA with low density, and high concentration, good quality and relatively low cost by chloroform/isoamyl alcohol method. [Conclusion] The chloroform/isoamyl alcohol extraction of genomic DNA is a more effective method of promoting and using in molecular studies on fresh pork liver.

关 键 词：猪肝 基因组DNA 氯仿/异戊醇抽提法 试剂盒法 比较 

分 类 号：S828[农业科学—畜牧学]