香椿乙醇提取物对糖尿病模型小鼠降血糖作用的初步研究  

Hypoglycemic Activity of Alcohol Extracts from Toon Sinensis in Diabetic Model Rats

作　　者：张俊芳[1,2] 温劲[1,2] 杨敏[1,2] 张美怡[3] 王定勇[4] 

机构地区：[1]广东省人民医院 [2]广东省医学科学院药学部,广州市510080 [3]广州市药品检验所二分所,广州市510250 [4]广东药学院,广州市510006

出　　处：《中国药房》2009年第18期1374-1375,共2页China Pharmacy

基　　金：广东省社会发展领域科技计划项目(63056)

摘　　要：目的:研究香椿乙醇提取物对糖尿病模型小鼠的降血糖作用及机制。方法:将四氧嘧啶用0.9%NS配成0.02g.mL-1溶液,按200mg·kg-1腹腔注射小鼠,5d后测血糖,空腹血糖>10.00mmol.L-1为高血糖模型小鼠。将模型小鼠随机分为4组,即模型(0.9%NS)、二甲双胍(70mg·kg-1)和香椿乙醇提取物高、低剂量组(剂量分别为1、0.5g·kg-1);另设正常组(蒸馏水),5组均每天灌胃1次,连续7d,末次给药后禁食24h,空腹眼眶静脉取血,测血糖和血清胰岛素水平。结果:与模型组比较,香椿乙醇提取物高、低剂量组血糖水平显著降低(P<0.05或P<0.01);血清胰岛素水平显著增高(P<0.05或P<0.01)。结论:香椿乙醇提取物对四氧嘧啶所致的高血糖模型小鼠有显著的降血糖作用,其作用机制可能与升高血清胰岛素水平有关。OBJECTIVE： To investigate the mechanism of the hypoglycemic activity of the alcohol extracts from Toon sinensis in diabetic model rats. METHODS： Alloxan was prepared into 0.02 g·mL^-1 solution with 0.9%NS then injected （intraperitoneally） into rats at a dosage of 200 mg·kg^-1.The blood glucose level was determined 5 days later, with hyperglycaemia model rats defined as rats with fasting blood glucose 〉 10.00 mmol·L^-1.Then the model rats were randomized to 4 groups： model （0.9 % NS）, metformin group （ 70 mg·kg^-1）, high dosage group of alcohol extracts from Toon sinensis （1g·kg^-1） and low dosage group of alcohol extracts from Toon sinensis （0.5 g·kg^-1）, besides, another normal group （distilled water） was established. The 5 groups were administered intragastrically with corresponding drugs q.d for 7 consecutive days, fasted for 24 hours after the last time of administration followed by blood sampling from orbital vein for determination of blood sugar and serum insulin levels. RESULTS： In rats treated with alcohol extract of Toon sinensis （either high dosage or low dosage）, the blood sugar level were significantly down- regulated（P（0.05 or P〈0.01） but the serum insulin level were significantly up- regulated as compared with model group（P 〈 0.05 or P〈0.01） .CONCLUSION： The alcohol extracts from Toon sinesis could effectively decrease the blood sugar in alloxan - induced diabetic rats and this might be-attributed to its up - regulation on serum insulin level.

关 键 词：香椿 乙醇提取物 糖尿病动物模型 降血糖 胰岛素 

分 类 号：R285[医药卫生—中药学] R972.6[医药卫生—中医学]