五氯酚厌氧生物降解体系中细菌的多样性分析  

Bacterial Diversity in PCP Anaerobic Biodegrading Consortium

作　　者：李向丽[1] 谭贵良[2,3] 陈坚[3] 张黎[4] 刘和[4] 

机构地区：[1]中山火炬职业技术学院生物医药系,中山528436 [2]广东省中山市质量计量监督检测所,中山528403 [3]江南大学工业生物技术教育部重点实验室,无锡214122 [4]江南大学环境与土木工程学院环境生物技术研究室,无锡214122

出　　处：《基因组学与应用生物学》2009年第3期481-485,共5页Genomics and Applied Biology

基　　金：国家自然科学基金资助项目(30640018);江苏省博士后科研资助计划项目(0701005C)共同资助

摘　　要：为了解五氯酚(PCP)降解过程中参与PCP降解的微生物多样性,本文应用16SrRNA基因克隆文库方法对PCP厌氧生物降解体系中细菌群落的组成和相对丰度进行了研究。结果表明,TM7类群的微生物在整个细菌群落中占有最大丰度(48.6%),检测到的序列与在三氯乙烯污染的地下水中检测的克隆子有一定的序列相似性(93.6%)。丰度位居第二的微生物类群为β-变形菌纲(Betaproteobacteria)细菌,其中的一些克隆子(10.8%)与脱氯微生物Dechlorosoma suillum具有极高的序列同缘性(99.7%)。此外,也检测到少数Clostridium属[厚壁菌门(Firmicutes)类群]的微生物。克隆文库中发现许多序列(占整个克隆文库的51.3%)与Gen Bank中已报道的序列具有较远的同源性(小于93.4%),它们可能代表新的微生物。本研究进一步拓宽了对PCP降解微生物多样性的认识。A method of 16S rRNA gene clone library was applied to investigate bacterial composition and relative abundance from pentachlorophenol （PCP） anaerobic biodegradating consortium, aiming to reveal bacterial diversity which capable of dechlorinating PCP. The result indicated that microorganisms affiliated to candidate TM7 dominated the clone library. They shared low sequence similarity （93.6%） with other clones detected from groundwater of contaminated soil by trichloroethylene. And, microorganisms belong to Betaproteobacteria comprised the sec ond abundance. Of them, some clones （10.8% of clone library） were phylogenetically associated （99.7%） with Dechlorosoma suillum, a dechlorinating microorganism. In addition, some microorganisms affiliated to genus Clostridium （Firmicutes group） were also retrieved. In particular, many new sequences （51.3% of clone library） were detected, since far associated （〈93.4%） with any known sequences in GenBank, they may represent new species. Thus, the present result extends our knowledge of PCP-dechlorinating microorganisms.

关 键 词：五氯酚 厌氧生物降解 16S RRNA 多样性 

分 类 号：X172[环境科学与工程—环境科学]