高粱与苏丹草EST-SSR标记的开发  

Development of EST-SSR Maker in Sorghum and Sudangrass

作　　者：卢杰[1,2] 詹秋文[2] 陆景标[1,2] 李杰勤[2] 王敏[1] 

机构地区：[1]安徽农业大学农学院,安徽合肥230036 [2]安徽科技学院植物科学学院,安徽凤阳233100

出　　处：《激光生物学报》2009年第3期389-394,400,共7页Acta Laser Biology Sinica

基　　金：科技部农业科技成果转化资金项目(No:2008GB2C300125);国家科技支撑计划项目(No:2008BADB3B3B10);安徽省“十一五”科技攻关计划重点项目(No:06013104B);安徽省高校学科拔尖人才基金项目(No:教秘人[2005]79号);安徽科技学院稳定人才专项(No:ZRC2009236)

摘　　要：从769条含SSR位点的高粱EST序列中设计了103对EST-SSR引物。用设计出的103对EST—SSR引物，对高粱品种TX623A和苏丹草品种S722进行了PCR扩增和多态性检测。结果表明：80对引物同时在两品种中扩出了条带，占引物总数的77．7％，20对引物在两品种中表现出多态性，占引物总数的19．4％，占能在两品种中扩出带引物的25．0％。研究结果表明根据高粱EST建立高粱与苏丹草EST-SSR标记是有效、可行的。One hundred and three （ 103 ） EST-SSR primers pairs were designed from 769 sorghum ESTs which contain SSR. The PCR amplification and polymorphism detection were conducted with sorghum variety TX623A and sudangrass variety S722 using 103 EST-SSR primer pairs. The results showed that 80 primers pairs give amplified clear bans in two varieties, accounting for 77.7 % of total primers, and 20 primers pairs showed polymorphism, accounting for 19.4 % of total primers, and accounting for 25.0 % of primer that could amplify clear bans in two varieties. These results indicated that it is an effective and feasible approach to develop sorghum and sudangrass EST-SSR markers based on ESTs in sorghum.

关 键 词：高粱 苏丹草 EST序列 EST-SSR标记 多态性 

分 类 号：S544[农业科学—作物学]