适宜猪背最长肌mRNA差异显示分析的2种总RNA提取方法的比较  

Comparison of Two Methods of RNA Isolation for mRNA Differential Display in Swine Longissimus Muscle

作　　者：李开桢[1] 曲亮[1,2] 黄春玲[1] 于传军[3] 吴建海[3] 王均顺 黄瑞华[1] 

机构地区：[1]南京农业大学动物科技学院,江苏南京210095 [2]江苏省家禽研究所,江苏扬州226000 [3]江苏省淮安市淮阴种猪场,江苏淮安223322

出　　处：《江苏农业学报》2009年第3期572-575,共4页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省高技术研究项目(BG2005304;BG2004317)

摘　　要：以苏淮白猪为材料提取RNA,比较异硫氰酸胍-酚-氯仿一步法与TRIzol试剂法提取RNA的效果。结果表明这2种方法提取的RNA经纯化之后均能够满足mRNA差异显示技术的需要,差别仅在于前者提取的RNA浓度稍高于后者,而后者更简便易行。Longissimus muscle of Suhuai white pig was selected to compare the effect of extracting total RNA by the methods of guanidinium isothioeyanate-phenol-ehloroform one step method and the method of TRIzol. The result showed that RNA extracted by either method could suffice the requirement of DDRT-PCR analysis. The concentration of RNA with the method of guanidinium isothiocyanate-phenol-chloroform one step method was slightly larger than that by the method of TRIzol. The method of TRIzol is simpler and faster than the method of guanidinium isothioeyanate-phenol-chlorofoim one step.

关 键 词：RNA 提取 DDRT—PCR 猪 背最长肌 

分 类 号：S828.1[农业科学—畜牧学]