蔡氏扶正消癥汤对胃癌SGC-7901细胞黏附分子表达的影响  

Effects of Prescription of Cai-shi-fu-zheng-xiao-zheng-Tang on the proliferation and cell adhesion molecule of human gastric carcinoma cell line SGC-7901 in vitro

作　　者：郎玮[1] 王海波[1] 吴建波[1] 章圣辉[1] 蔡慎初[1] 尹丽慧[1] 

机构地区：[1]温州医学院附属第一医院中医科,浙江温州325000

出　　处：《温州医学院学报》2009年第4期342-344,共3页Journal of Wenzhou Medical College

基　　金：浙江省科技计划基金资助项目(2008C30010)

摘　　要：目的:探讨蔡氏扶正消癥汤对人体外生长胃癌细胞SGC-7901表面黏附分子的影响以及作用的计量和效应关系。方法:流式细胞仪检测细胞膜表面E-Cadherin,CD44,CD44-v6和CD54的表达。结果:蔡氏扶正消癥汤作用48h后,SGC-7901细胞膜表面的E-Cadherin表达增高,CD44、CD44-V6和CD54表达减少。随着药物剂量增加,E-Cadherin表达阳性细胞表达逐渐升高;CD44、CD44-v6、CD54表达的阳性细胞比率逐渐下降,抑制率也逐渐升高。结论:蔡氏扶正消癥汤在抑制人胃癌细胞SGC-7901体外增殖的基础上,能够增加胃癌细胞SGC-7901表面E-Cadherin的表达,减少胃癌细胞SGC-7901表面CD44,CD44-V6和CD54的表达,并在计量和效应间存在联系,说明蔡氏扶正消癥汤能阻止胃癌SGC-7901细胞对邻近正常组织的浸润及远处转移,从而发挥抗癌作用。Objective： To investigate the effects as well as dose and effects relationship of Prescription of Cai-shi-fu-zheng-xiao-zheng-Tang on the proliferation and cell adhesion molecule changes of human gastric carcinoma cell line SGC-7901 in vitro. Methods： Flow cytometry was used to analyze the expression of cell adhesion molecules, including E-Cadherin, CD44, CD44-V6 and CD54. Results： Cai-shi-fu-zheng-xiao-zheng-Tang inhibited the proliferation of SGC-7901 cells in a dose- and time-dependent manner, with the expression of E-cadherin increased concomitant with CD44, CD44- V6 and CD54 depressed. Conclusion： Cai-shi-fu-zheng-xiao-zheng-Tang plays its anti-cancer function through inhibit proliferation and effect on cell adhesion molecules.

关 键 词：中药方剂 胃癌细胞 E-钙黏蛋白 CD44 CD54 

分 类 号：R730.52[医药卫生—肿瘤] R735.2[医药卫生—临床医学]