NGAL基因克隆载体的构建与鉴定  

Construction and identification of the cloning vectors for neutrophil gelatinase-associated lipocalin gene

作　　者：王彩纹[1] 王小中[1] 

机构地区：[1]南昌大学第二附属医院检验科,330006

出　　处：《实验与检验医学》2009年第3期217-218,296,共3页Experimental and Laboratory Medicine

基　　金：江西省卫生厅课题资助(项目编号20061109)

摘　　要：目的构建中性粒细胞明胶酶相关脂质运载蛋白(neutrophil gelatinase-associated lipocalin,NGAL)基因克隆载体。方法RT-PCR扩增宫颈癌Hela细胞NGAL cDNA片断,将纯化后PCR产物插入pGEM-T载体,构建重组质粒,重组子通过琼脂糖电泳、特异性内切酶切割及测序予以鉴定。结果成功构建了NGAL基因克隆载体2个。结论为NGAL基因的定量检测提供了基本实验条件。Objective To construct the cloning gene vectors of neutrophil gelatinase-associated lipocalin （NGAL） gene for its quantitative detection. Methods Recombinant plasmid was constructed by NGAL cDNA fragment from Hela cell into pGEM-T vector by RT-PCR. The recombined clones were identified by agarose gel electrophoresis, restriction endonuclease digesting and biological analysis. Results Two cloning vectors had been construsted. Conclusions These cloning vectors offered the basic experimental conditions for quantitative detection of NGAL.

关 键 词：中性粒细胞明胶酶相关脂质运载蛋白 (neutrophil GELATINASE - ASSOCIATED lipocalin  NGAL) 基因 载体构建 

分 类 号：R735[医药卫生—肿瘤] R730.43[医药卫生—临床医学]