蟾蜍灵联合ATRA诱导HL-60细胞分化并上调Syk表达  

The expression of Syk was up-regulated during the differentiation of HL-60 cell induced by bufalin and all-traus retinoic acid(ATRA)

作　　者：肖家雯[1] 曲秀娟[1] 刘云鹏[1] 马艳菊[1] 侯科佐[1] 李迎春[1] 张晔[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤内科,辽宁沈阳110001

出　　处：《现代肿瘤医学》2009年第7期1227-1229,共3页Journal of Modern Oncology

基　　金：辽宁省科学技术厅基金资助项目(2004225004-11)

摘　　要：目的:观察低剂量蟾蜍灵、全反式维甲酸(All-trans retinoic acid,ATRA)单药或联合应用对HL-60细胞增殖及分化的影响,同时检测上游通路Syk表达的变化。方法:流式细胞仪技术检测CD11b表达;West-ern Blot检测Syk和Tubulin表达。结果:与对照组相比,低剂量蟾蜍灵、ATRA或联合用药不同程度地抑制HL-60细胞增殖,同时蟾蜍灵联合ATRA以时间依赖性的方式诱导HL-60细胞分化,并伴有Syk表达上调,但是Syk的酪氨酸磷酸化并未受影响。结论:Syk表达上调可能参与调节低剂量蟾蜍灵联合ATRA对HL-60细胞的分化诱导作用。Objective：To investigate the changes of proliferation and differentiation,the changes of spleen protein tyrosine kinase Syk during HL - 60 cells differentiation induced by low dose of bufalin and ATRA. Mehtods： The expression of CD11 b was detected using flow eytometery assay. The expressions of Syk and Tubulin were tested using Western Blot. Results： Cells treated with bufalin, ATRA and the combination of bufalin and ATRA showed strong growth inhibition, compared to the control cells. Furthermore the eombination of 5 nmot/L bufalin and 30 nmol/L AT- RA induced HL - 60 cells differentiation in a time - dependent manner. Meanwhile the expression of Syk was also up - regulated in a time - dependent manner, but the tyrosine phosphorylation of Syk was not affected. Conclusion： Up - regulation of Syk might be involved in the regulation of HL -60 cells differentiation.

关 键 词：SYK 分化 ATRA 

分 类 号：R730.53[医药卫生—肿瘤]