宫颈癌P_(53)基因突变的检测  

Detection of P_(53) gene mutation in cervical carcinoma

作　　者：陈卫[1] 李文惠[1] 

机构地区：[1]兰州医学院生化教研室

出　　处：《兰州医学院学报》1998年第2期10-11,14,共3页Journal of Lanzhou Medical College

基　　金：甘肃省自然科学基金

摘　　要：目的：探讨抑癌基因P（53）突变与宫颈癌发生发展之间的关系，并为该病的临床检测提供一种分子生物学方法。方法：应用多聚酶链反应-单链构象多态性（PCR－SSCP）分析方法对宫颈癌及慢性宫颈炎组织中P（53）基因5－6外显子的突变进行了检测。结果：35例宫颈癌组织中有2例出现突变，突变率为5．71％；而作为对照的慢性宫颈炎组织中无一例出现突变。结论：宫颈癌的发生发展与抑癌基因P（53）－6外显子的突变有关；此方法可有效地检测抑癌基因P（53）的突变。Objective: To investigate the relationship between the progression and development in cervical carcinoma and the tumor suppressive gene P53 and to suPPly a melecular biological method Of clinical detection in this disease. Methods By using PCR -SSCP technology detected the mutations of exon 5 -6 in P53 gene in cervical carcinoma and chronic cervicitis tissues. Results The mutation was identitied in 2 Out of 35 cases of cervical carcinoma tissues. The mutation rate were 5 .71%, The exon 5 -6 in P53 gene were not detected mutation in chronic cervicitis. Conclusion PCR -SSCP method is effective to deteCt gene mutation. P53 gene mutation was relative in the development and progression of cervical carcinoma.

关 键 词：宫颈癌 P53基因 聚合酶链反应 基因突变 

分 类 号：R737.330.2[医药卫生—肿瘤]