YP3:食用菌榆黄蘑中新的植物病毒抑制物蛋白(英文)  

YP3:a Novel Plant Virus Inhibitory Protein from Mushroom Pleurotus citrinopileatus

作　　者：吴丽萍[1] 曹郁生[1] 吴祖建[2] 林奇英[2] 谢联辉[2] 

机构地区：[1]南昌大学生命科学与食品工程学院,南昌330047 [2]福建农林大学植物病毒研究所,福州350002

出　　处：《天然产物研究与开发》2009年第3期371-376,共6页Natural Product Research and Development

基　　金：supported by the Key Projectof Fujian Province (No.2006NZ0002-2);Science and Technology Founda-tion of Educational Department of Jiangxi Province([2007]31)

摘　　要：食用菌中含有多种抗病毒蛋白,可用于植物保护。采用硫酸铵分级沉淀、DEAE-纤维素离子交换层析和SephacrylTMS-200凝胶层析方法,从食用菌榆黄蘑新鲜子实体中提取到一单亚基蛋白,命名为YP3。利用SDS-聚丙烯酰胺不连续凝胶电泳初步估计其分子量大约为27.6kDa。氨基酸组成分析表明该蛋白非常类似其它的抗植物病毒蛋白,并且几乎不含糖。其N-末端序列为NRDVAACARFIDDFCDTLTP,在GenBank中没有找到同源序列。浓度为0.24mg/L时蛋白YP3对烟草花叶病毒(TMV20mg/L)侵染心叶烟的抑制率为50%。同时还发现YP3对供试的细菌和真菌没有抑制活性,对胃癌细胞株MGC-803、肝癌细胞株SMMC-7721、肺癌细胞株SPC-A1的细胞增殖具有一定的抑制作用,其IC50大约为20mg/L。Many proteins from mushrooms can be used as potential antiviral agents, and in plant protection. An antiviral protein （YP3） was isolated from fruiting bodies of the fungus Pleurotus citrinopileatus with a procedure involving ammonium sulfate precipitation, ion-exchange chromatography on DEAE-cellulose, and gel filtration on Sephacryl^TM S-200 High Resolution. The molecular weight of protein was determined to be about 27.6 kDa by Sodium dodecyl sulfate -polyacrylamide gel electrophoresis （SDS-PAGE）. The amino acid analysis indicated that it was similar to some other antiviral proteins. The protein was almost not sacchariferous. The sequence of N-terminal of YP3 was NRDVAACARFIDDFCDTLTP,which had no homology with other sequences in GenBank. YP3 at 0.24 mg/L achieved 50.0% inhibition of Tobacco mosaic virus （TMV 20 mg/L） lesions in Nicotiana glutinosa leaves;YP3 is inactive to some bacteria and fungi tested; YP3 inhibited stomach cancer cell line MGC-803, liver cancer cell line SMMC-7721 and lung cancer cell line SPC-Alwith an IC50 of about 20 mg/L.

关 键 词：食用菌 榆黄蘑 蛋白 抗病毒活性 

分 类 号：Q939.99[生物学—微生物学] R078.7[医药卫生]