NGF与癫痫后大鼠海马损伤修复及苔藓纤维发芽关系的研究  

The correlation along nerve growth factor,the repairing of neuron damage and mossy fibers sprouting in rat hippocampus after lesion

作　　者：郑金瓯[1] 黄颖[1] 梁志坚[1] 吴原[1] 余璐[1] 

机构地区：[1]广西医科大学第一附属医院神经内科,广西南宁530021

出　　处：《中风与神经疾病杂志》2009年第3期270-273,共4页Journal of Apoplexy and Nervous Diseases

基　　金：广西科学基金项目(桂科自0640118)

摘　　要：目的探讨神经生长因子(NGF)与癫痫后大鼠海马损伤修复及与苔藓纤维发芽(MFS)的关系。方法大鼠侧脑室内分别注入NGF正、反义寡核苷酸和磷酸盐缓冲液,并使用红藻氨酸(KA)致痫后分别在48h、72h、1w和4w采用普通病理方法、免疫组化和Timm’s染色法对鼠脑片进行研究。结果KA致痫后48h大鼠海马NGF蛋白表达已可见显著升高,持续较高水平表达至少4w,并伴随大鼠海马明显MFS;给予NGF反义寡核苷酸能够减少NGF蛋白表达和MFS过程,但神经元损伤更为严重;大鼠行为学观察各癫痫组未见明显差异。结论KA致痫后大鼠海马NGF蛋白表达升高并与MFS同时共存,但可被侧脑室内注入NGF反义寡核苷酸有效阻止,提示NGF可促进癫痫后大鼠海马损伤修复及MFS过程。Objective To explore the correlation along nerve growth factor, the repairing of neuron damage and mossy fibers sprouting （MFS） in rat hippocampus after leison. Methods Antisense or sense oligonucleotide to NGF and/ or PBS buffer was injected into the ventricles of rats of temporal lobe epilepsy model induced by KA and so as the control rats,then they were executed at 48h,72h, 1 w and 4w after lesion respectively. Immunohistochemical, pathobiology staining and Timm＇ s silver sulfide staining were used. Results The NGF-like protein expression increased at 48h after lesion, then the protein expression＇ s peak was at 72h and kept at a high level till about 4w, and with positive correlation with MFS. Timm＇ s staining showed that multiple aberrant Timm＇ s granules were found in the hippocampus of the KA model intracere- broventricularly injected with sense oligonucleotide to NGF and PBS. But antisense oligonucleotide to NGF alleviated these states. No obvious discrepancy of behavior between the epileptic rats injected antisense oligonucleotide and the sense oligonucleotide ones. Conclusions The NGF-like protein expression increased with time-dependence after lesion and coexisted with MFS, but antisense oligonucleotide to NGF could alleviate these states. It hinted that NGF can promote the repairing of neuron damage and mossy fibers sprouting （MFS） in rat hippocampus afler leison.

关 键 词：神经生长因子 癫痫 反义寡核苷酸 苔藓纤维发芽 神经元损伤 

分 类 号：R742.1[医药卫生—神经病学与精神病学]