实验性自身免疫性脑脊髓炎模型的建立和长期观察研究  

The establishment and a long term study of the mice model of experimental autoimmune encephalomyelitis

作　　者：吴志英[1] 赵振华[1] 林毅[1] 苏宗权[1] 陈枝挺[1] 王柠[1] 

机构地区：[1]福建医科大学附属第一医院神经内科,福建福州350005

出　　处：《中风与神经疾病杂志》2009年第3期300-302,共3页Journal of Apoplexy and Nervous Diseases

基　　金：福建省自然基金重点项目(C0520002);福建省卫生厅青年科研基金资助项目(2006-1-3);人事部留学人员科技活动项目择优资助经费

摘　　要：目的建立实验性自身免疫性脑脊髓炎小鼠模型(EAE)并长期观察研究。方法C57BL/6小鼠30只,随机分为EAE模型组、PBS对照组和正常对照组。应用神经功能评分进行临床评估,通过HE和髓鞘染色观察组织病理变化。结果小鼠在诱导后的12±3d急性起病,16±2d内达到高峰,严重度评分为3.2±0.6分。半年观察期内复发1次,复发率为25%。光镜下可见EAE组以脊髓组织病变为主,表现为大量炎性细胞浸润和白质脱髓鞘。结论采用MOG35-55诱导C57BL/6小鼠建立的模型既往被认为是一种慢性迁延EAE模型,本研究通过长期观察发现其存在缓解复发现象。Objective To establish a mice model of experimental autoimmune encephalomyelitis （EAE） and perform a long term study. Methods C57BL/6 mice were immunized with 300μg MOG35-55 in complete Freund＇ s adjuvant （CFA） to establish EAE model in EAE group （n = 10 ）. Mice in adjuvant group（ n = 10 ）were treated with CFA without MOG35-55 and control group（ n = 10）were treated with normal saline. The pathologic changes of the central nervous system were studied by HE staining and myelin staining. Results The clinic symptoms of EAE were present in the 12 ± 3th day post-immunization, and went to the peek in the 16 ±2 th day post-immunization. The severity score was 3.2 ± 0.6. One relapse was observed in the term of 6 months, and the rate was 25%. Light microscopy showed there were abundant inflammatory cells infiltrated especially in spinal cord tissues in EAE mice,with evident demyelination in white matter. Conclusion The relapse of this EAE model was observed in the study, though it was believed to be a chronic persistent model without relapse.

关 键 词：多发性硬化 实验性自身免疫性脑脊髓炎 MOG35-55 C57BL/6 

分 类 号：R744.51[医药卫生—神经病学与精神病学]