地龙注射液HPLC指纹图谱的研究  

HPLC fingerprints of Dilong injection

作　　者：詹云丽[1] 黄璐敏[1] 翁嘉[1] 黄丹莹[1] 李晓蒙[2] 裘建社 

机构地区：[1]惠州市药品检验所,广东惠州516003 [2]广东药学院药科学院,广东广州510006 [3]广东省博罗先锋药业集团有限公司,广东惠州516100

出　　处：《广东药学院学报》2009年第3期245-248,共4页Academic Journal of Guangdong College of Pharmacy

摘　　要：目的建立地龙注射液中游离氨基酸的HPLC指纹图谱。方法采用2,4-二硝基氟苯柱前衍生化法,用HPLC法进行梯度洗脱分析地龙注射液中游离的氨基酸。结果通过优化衍生条件,得到分离度、重复性均较好的HPLC指纹图谱,确定了14个共有峰,以缬氨酸为参照峰,用中药指纹图谱相似度软件计算不同批次样品的相似度均在0.97以上。结论本研究建立的指纹图谱,可用于地龙注射液的质量控制。Objective To establish the HPLC fingerprints of free amino acids in Dilong injection. Methods 1-fluoro-2,4-dinitrobenzene was used for the pre-column derivation of amino acids in the Dilong injection, then analyzed with acetonitrile-DMF-sodium acetate gradient elution. Results The HPLC fingerprints were well separated. Fourteen common peaks were obtained. The similarities among different batches of samples were all above 0.97 when using valine as reference peak. Conclusion This method could be used for the quality control of Dilong injection.

关 键 词：地龙注射液 指纹图谱 HPLC 氨基酸 

分 类 号：R286[医药卫生—中药学]