应用基因组原位杂交技术鉴定抗黄矮病小麦新种质  

Analysis of the Chromosome Constitution of Wheat Lines Resistant to Barley Yellow Dwarf Virus  by Genomic in situ Hybridization

作　　者：张增艳[1] 马有志[1] 辛志勇[1] 陈孝[1] 武东亮[1] 林志珊[1] 

机构地区：[1]中国农业科学院作物育种栽培研究所

出　　处：《中国农业科学》1998年第3期1-4,共4页Scientia Agricultura Sinica

基　　金：国家"863"计划项目;国家自然科学基金

摘　　要：利用生物素（ｂｉｏｔｉｎ－１６－ｄＵＴＰ）标记的中间偃麦草（Ｔｈｉｎｏｐｙｒｕｍｉｎｔｅｒｍｅｄｉｕｍ）基因组ＤＮＡ作探针，以未标记的普通小麦中国春基因组ＤＮＡ作封阻ＤＮＡ（ｂｌｏｃｋｉｎｇＤＮＡ），对３个抗黄矮病小麦新种质的体细胞染色体进行分子原位杂交。结果表明：抗性源于Ｌ１的抗黄矮病小麦新种质Ｙｗ６４２为小片段易位系，含４０条小麦染色体和２条小麦－中间偃麦草易位染色体，易位的中间偃麦草染色体片段位于小麦染色体的端部；染色体配对和抗性分析表明该种质为纯合易位系。抗性源于无芒中４的小麦新种质Ｈｗ２４０和Ｙｗ０６０遗传构成不同：Ｙｗ０６０为易位系，含４０条小麦染色体和２条小麦－中间偃麦草易位染色体；Ｈｗ２４０为代换易位系，含３８条小麦染色体、２条中间偃麦草染色体和２条小麦－中间偃麦草易位染色体。在这２个种质中，易位的中间偃麦草染色体片段均位于小麦染色体端部。The chromosome constitution of three lines (Yw642, Yw060 and Hw240) with BYDV resistance was analyzed by genomic  in situ hybridization (GISH) in which biotin labelled total genomic DNA of Thinopyrum intermedium was used as probe and the unlabelled total DNA of common wheat cv. Chinese Spring as blocking DNA. The results showed that the lines Yw642 and Yw060 were translocation lines. The chromosome composition of these two lines was composed of 1 pair of wheatTh.intermedium  translocation chromosomes and 20 pairs of wheat chromosomes. In both cases, the translocation chromosome segment of Th.intermedium was located at distal end of wheat chromosome arm. The line Hw240 was a substitution /translocation line in which chromosome composition was formed by 38 wheat chromosomes, 2 wheatTh.intermedium translocation chromsomes and 2 Th.intermedium chromosomes.

关 键 词：基因组原位杂交 小麦 黄矮病抗性 易位系 

分 类 号：S512.102[农业科学—作物学]