超高效液相色谱-串联质谱法快速检测鳗鱼中磺胺类、喹诺酮类、四环素族抗生素药物残留  

Simultaneous Determination of Qunolones, Sulfonamides and Tetracyclines Residues in Eel Using Solid Phase Extraction (SPE) and Ultra-performance Liquid Chromatography-Electrospray Tandem Mass Spectrometry (UPLC-MS-MS)

作　　者：刘正才[1] 杨方[1] 余孔捷[1] 李耀平[1] 林永辉[1] 张琼[1] 刘素珍[1] 

机构地区：[1]福建出入境检验检疫局,福建福州350001

出　　处：《食品科学》2009年第14期167-170,共4页Food Science

基　　金：国家质量监督检验检疫总局科技计划项目(2007IK147);福建省科技计划重点项目(2007Y001)

摘　　要：建立鳗鱼中15种磺胺、3种喹诺酮类以及4种四环素族抗生素残留的超高效液相色谱-串联质谱同时测定方法。采用酸性EDTA-Mcllvaine缓冲液提取,经HLB固相萃取小柱富积、净化后进行UPLC分离,电喷雾串联质谱进行检测,多离子反应监测(MRM)模式定量。添加水平为5、10、20μg/kg时各种化合物的平均回收率范围为72.4%～97.3%,RSD值均小于14.4%。该法简单、快速、灵敏,适用于鳗鱼中多磺胺组分、喹诺酮及四环素族组分残留的同时测定。A robust and sensitive UPLC-MS-MS method was developed for the simultaneous determination of residues of 15 sulfonamides, 3 fluoroqninolones and 4 tetracylines in eel. The target compounds were extracted with acidic EDTA-Mcllvaine buffer solution, and enriched and cleared up using a hydrophilic-lipophilic balance （I-ILB） SPE cartridge. After seperation on UPLC BEH C18, the analytes were detected using a tandem mass spectrometry and quantified in multiple reaction monitoring （MRM） mode. At the spike levels of 5, 10 and 20 μg/kg respectively, the average recoveries for all the compounds were 72.4%-97.3%, and the variation coefficients were all under 14.4%. This method is simple, fast, sensitive, and particular for the simulaneous determination of sulfonamides, fluoroquinolones and tetracylines in eel.

关 键 词：超高效液相色谱串联质谱法(UPLC-MS-MS) 磺胺类 喹诺酮类 四环素族 鳗鱼 

分 类 号：TS207.53[轻工技术与工程—食品科学]