气相色谱-质谱法测定茶叶中36种农药残留  

Simultaneous Determination of 36 Pesticide Residues in Tea by GC-MS

作　　者：蒋俊树[1,2] 赵彬[2] 周莉[2] 

机构地区：[1]安徽国家农业标准化与监测中心,安徽合肥230051 [2]安徽大学化学化工学院,安徽合肥230039

出　　处：《食品科学》2009年第14期276-280,共5页Food Science

基　　金：安徽省科技攻关项目(08010302216);国家标准制修订项目(20068008-T-469)

摘　　要：建立气相色谱-质谱(GC-MS)联用技术同时测定茶叶中36种农药残留方法。茶叶样品用加速溶剂提取(ASE),凝胶渗透色谱(GPC)净化,气相色谱-质谱法(GC-MS)的选择离子监测(SIM)方式检测,外标法定量。该方法同时测定了茶叶中36种农药残留,在0.05、0.2、1.0mg/kg3个添加水平,平均加标回收率为67.8%～110.2%,相对标准偏差(RSD)为1.72%～8.89%,36种农药的检出限最低为5μg/kg,最高为50μg/kg。该方法快速、灵敏、准确,各项技术指标均满足国内外农药残留检测限量标准要求,能够很好的应用于茶叶中痕量农药的残留测定。A gas chromatography-mass spectrometry （GC-MS） method was developed for the simultaneous detection of 36 pesticide （including 9 kinds of pyrethroid pesticides, 12 kinds of organochlorine pesticides and 15 kinds of organophosphorus pesticides） residues in tea. The method was based on accelerated solvent extraction （ASE） with a mixture of acetonitrile and dichloromethane （1：1, V/V） and gel permeation chromatography （GPC） cleanup followed by GC-MS analysis of the extracts in selected ion monitoring （SIM） mode. The external standard method was adopted for quantitative analyses. At the three spike levels of 0.05, 0.2 and 1.0 mg/kg, mean recoveries for the 36 pesticides were between 67.8% and 110.2% with a relative standard deviation （RSD） ranging from 1.72% to 8.89%. The limits of detection were between 5μg/kg and 55 μg/kg. This method is rapid, sensitive and accurate and can meet the demands for determining pesticides in tea samples.

关 键 词：气相色谱质谱联用法 加速溶剂提取(ASE) 凝胶渗透色谱(GPC) 茶叶 农药残留 

分 类 号：R155.51[医药卫生—营养与食品卫生学]