秦皇岛市食品中O157:H7大肠杆菌检测分析与探讨  

Analysis of monitoring EHEC O157:H7 in food in Qinhuangdao City

作　　者：冯艳洁[1] 张海滨[1] 

机构地区：[1]河北省秦皇岛市疾病预防控制中心,河北秦皇岛066000

出　　处：《中国卫生检验杂志》2009年第7期1630-1631,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:对秦皇岛市食品中O157:H7大肠杆菌的污染状况进行监测并分析。方法:用改良EC肉汤增菌,采用O157免疫磁珠分离技术,接种柯玛嘉和青岛海博O157显色平板,血清和生化系列证实。结果:220份食品中检出O157:H7大肠杆菌2株,分别为牛肉1株,羊肉1株,检出率0.91%。检出可疑O157:H7大肠杆菌2株,怀疑为L型O157:H7大肠杆菌。结论:秦皇岛市食品中存在O157:H7大肠杆菌的污染,需要对该菌进行长期监测。同时加强L型O157:H7大肠杆菌的检测鉴定。Objective：To monitor and analyze contamination of food by E. coli O157：H7 in Qinhuangdao City. Methods：Enrich enterobacterium with EC Broth, adopt O157 immunomagneti6 microspheres isolation technique, inoculate Qiangdao Haibo O157 colorable culture medium, prove through serology and bio -chemical experiments. Results：We detected 2 strains of E. coli O157 ：H7 in 220 shares of food samples, one was found on beef sample and another on mutton sample, the detectable rate was 0. 91%. We detected 2 strains of suspected E. coli O157：H7 that may be L coli O157 ：HT. Conclusion ：There is contamination of food by E. coli O157 ： H7 in Qinhuangdao, which should be monitored in long term. The monitoring and identification of L E. coli O157 ：H7 should be enhanced.

关 键 词：O157:H7大肠杆菌 污染 食品 监测 

分 类 号：R155.5[医药卫生—营养与食品卫生学]