高效石油降解菌的筛选及初步鉴定  

Isolation and Preliminary Identification of Oil-Degrading Microorganism

作　　者：王伟平[1] 李伟 邱雁临[1] 张华山[1] 彭晓斌[1] 

机构地区：[1]湖北工业大学生物工程学院,湖北武汉430068 [2]武汉市汉江石油物资技术开发有限责任公司,湖北武汉430051

出　　处：《化学与生物工程》2009年第7期64-66,共3页Chemistry & Bioengineering

摘　　要：经过以石油烃为唯一碳源的选择性培养基平板初筛和三角瓶发酵复筛,采用紫外分光光度法测定石油降解率,从江汉油田和冀东油田石油污染的土壤和水体中,筛选出有降解石油能力的微生物9株。其中3株细菌(X-1,X-2,X-3)降解石油能力较高,X-1菌株的石油降解率最高达64.28%。根据形态学观察和部分生理生化特征初步鉴定,该菌为节杆菌属(Arthrobacter sp.)。Nine strains which could degrade crude oil efficiently were isolated from oil-containing soil or water of Jianghan Oil Field and Jidong Oil Field,and were screened on the plates and flasks respectively in the selective medium with oil as sole carbon source. The results showed that three bacteria （X-1,X-2,X-3） had rela- tively high capacity of oil degradation and the best one was strain X-l, with degradation rate of oil as high as 64.28 %. It was belong to Arthrobacter sp. by preliminary identification through configuration observation and physiological and biochemical characteristics.

关 键 词：石油污染 紫外分光光度法 石油降解菌 降解率 

分 类 号：X172[环境科学与工程—环境科学]