亚硝酸降解法制备低分子肝素(LMWH)的工艺参数优化与确定  

The parameter optimization and definition of LMWH preparation technology by nitrous acid

作　　者：张丽萍[1,2] 李雷刚[2] 马中苏[1] 

机构地区：[1]吉林大学生物与农业工程学院长春,130022 [2]黑龙江八一农垦大学食品学院,大庆163319

出　　处：《药物分析杂志》2009年第7期1148-1151,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：黑龙江省科技原科技攻关项目NOGB06B0403-3

摘　　要：目的:研究低分子肝素(LMWH)制备的最佳条件。方法:利用亚硝酸降解法,通过单因素试验与二次旋转回归组合试验,研究不同温度、时间、pH和亚硝酸浓度等条件下LMWH的相对分子质量大小、分布及与标准品相对分子质量之差。结果:在反应时间4h、温度25℃左右、亚硝酸浓度0.6%、pH2.6时,相对分子质量大小符合中国药典规定,相对分子质量小于8000的成分含量为89.95%。结论:利用亚硝酸降解肝素制备LMWH的方法可行。Objective：Study on the optional preparation conditions of the low molecular w Method ： Degradation of heparin by nitrous acid, studied on different temperature, time, pH eight heparin, LMWH）. and nitrous acid concentrations, researched single factors experiment and dual quadratic rotary combinational design, measured the molecular and distribution of relative molecular mass. Results： Research showed that the degradation time was 4 h, temperature was about 25 ℃, pH2. 6, nitrous acid concentration was 0. 6% , under those conditions, both the preparation of relative molecular mass and the distribution of relative molecular mass were corresponding with general rule of Chinese Pharmacopoeia. The distribution of relative molecular mass less than 8000 was 89.95%. Conclusion： The method of degradation of heparin by nitrous acid for the preparation of LMWH is feasible.

关 键 词：低分子肝素(LMWH) 亚硝酸 降解 制备 

分 类 号：R917[医药卫生—药物分析学]