广西肝癌高、低发区CYP3A5基因多态性的对比  

Comparative analysis on the genetic polymorphisms of CYP3A5 between hepatocellular carcinoma high and low incidence area in Guangxi Zhuang Autonomous Region

作　　者：卢凤美[1] 刘东璞[1] 孟庆媛[1] 郭梦凡[1] 冯震博[2] 

机构地区：[1]黑龙江省佳木斯大学司法鉴定中心佳木斯大学基础医学院病理法医教研室,黑龙江省佳木斯市154007 [2]广西医科大学基础医学院病理教研室,广西壮族自治区南宁市530021

出　　处：《世界华人消化杂志》2009年第15期1570-1574,共5页World Chinese Journal of Digestology

摘　　要：目的:研究广西肝癌高发区和低发区人群中CYP3A5基因多态性,以探讨CYP3A5基因多态性与肝癌易感性的关系.方法:收集广西医科大学第一附属医院2005/12-2006/09经病理确诊的72例肝细胞癌(HCC)患者的肝癌组织及其癌旁组织,患者分别来自于肝癌高发区(黄曲霉素高污染区)和低发区.12例血管瘤的瘤旁肝组织作为正常对照.用PCR-RFLP法检测CYP3A5在上述标本中的多态性.结果:广西肝癌高发区患者CYP3A5*1等位基因频率和基因型比(CYP3A5*1/*1+CYP3A5*1/*3)与广西肝癌低发区患者比较,差异有统计学意义(P<0.05);肝癌高发区人群与正常对照组比较,差异有统计学意义(P<0.05);低发区与正常对照组之间比较,差异无统计学意义.结论:CYP3A5可能参与黄曲霉毒素B1(AFB1)相关性HCC的发生.AIM： To investigate the relationship between genetic polymorphism of CYP3A5 and HCC affectability through analysis of two groups of patients coming from Guangxi HCC highincidence area and HCC low-incident area. METHODS： We had collected 72 cases of HCC tissues and HCC-adjacent tissues from the First Affiliated Hospital of Guangxi Medical University during 2005-12/2006-09, and all the cases, who came from Guangxi HCC high-incidence area and HCC low-incidence area respectively, had been pathologically confirmed. At the same time, we used 12 cases of hepatic angioma-surrounding tissue as the normal control group. PCR-RFLP assay was used to analyze CYP3A5 genetic polymorphism of the above-mentioned samples. RESULTS： There were statistic differences between the population with CYP3A5＊1 allele frequency and the proportion of （CY- P3A5＊1/＊1 ＋ CYP3A5＊1/＊3） genotypes of Guangxi HCC high-incidence area and the population of Guangxi HCC low-incidence area （P 〈 0.05）. There were statistic differenc- es between the population of Guangxi HCC high-incidence area and the population of the normal control group （P 〈 0.05）. There were no statistic differences between Guangxi HCC low-incidence area population and the normal control group. CONCLUSION： CYP3A5 might take part in the morbidity of AFB1 related HCC.

关 键 词：CYP3A5 遗传多态性 黄曲霉毒素B1 肝细胞癌 聚合酶链反应-限制性片段长度多态性 

分 类 号：R735.7[医药卫生—肿瘤]