选育羊栖菜与野生羊栖菜中褐藻胶与褐藻糖胶组成分析  

Comparison analysis of alginate and fucoidan between artificial and wild brown alga Hizikia fusiforme

作　　者：王培培[1] 于广利[1] 杨波[1] 赵峡[1] 刘吉东[2] 段德麟[2] 李生尧 

机构地区：[1]中国海洋大学医药学院,山东青岛266003 [2]中国科学院海洋研究所,山东青岛266071 [3]浙江洞头县鹿丰羊栖菜研究所,浙江洞头325700

出　　处：《中国海洋药物》2009年第3期39-43,共5页Chinese Journal of Marine Drugs

基　　金：国家科技部课题(2007DFA30980和2007AA09Z445);国家自然科学基金(40618001和N_CUHK438/06);山东省农业良种工程课题资助

摘　　要：目的比较选育羊栖菜(H.fusiformis)与野生羊栖菜中褐藻胶与褐藻糖胶的组成差异。方法采用高效凝胶渗透色谱法(HPGPC)分析多糖相对分子量,用核磁共振氢谱法测定褐藻胶中G/M比,并用高效离子色谱(HPIC)法分析褐藻糖胶中单糖组成。结果选育羊栖菜和野生羊栖菜中褐藻胶和褐藻糖胶含量分别为29.23%,1.89%和32.18%,2.40%;褐藻胶中G/M比分别为2.62和1.05;褐藻糖胶均含有8种单糖,分别为岩藻糖,半乳糖,甘露糖,葡萄糖醛酸,葡萄糖,木糖,葡萄糖胺和甘露糖醛酸,其摩尔比分别为34.62,18.55,14.17,12.72,10.60,5.79,2.42,1.13及34.76,19.26,10.21,12.70,5.49,10.22,0.70,6.66。结论选育羊栖菜褐藻胶中古罗糖醛酸含量以及褐藻糖胶中葡萄糖,甘露糖和氨基葡萄糖含量均明显高于野生羊栖菜。Objective To compare the difference of alginate and fucoidan between artificial and wild brown alga H. fusiforme. Methods The relative molecular masses of polysaccharides were analyzed by high performance gel permeation chromatography（HPGPC）, the G/M ratio of alginate was determined by ^1H-NMR, and the monosaccharide compositions of fucoidan were tested by high performance ion chromatography（HPIC）. Results The contents of alginate and fucoidan in artificial and wild H. fusiforme were 29.23%, 1.89% and 32. 18%, 2.40 %, respectively. The G/M ratio in alginate were 2.62 and 1.05, separately. There are eight kinds of monosaccharides in fucoidan, and the molar ratio of fucose, galactose, mannose,glucuronic acid, glucose, xylose, glucosamine and mannuronic acid in the above algae were 34.62,18.55,14. 17,12.72,10.60,5.79,2.42,1. 13 and 34.76,19.26,10.21,12.70, 5.49,10.22,0.70,6.66, respectively. Conclusion The contents of guluronic acid in alginate and the glucose,mannose and glucosamine of fucoidan in artificial H. fusiforme were signif icant higher than that in wild type.

关 键 词：羊栖菜 褐藻胶 褐藻糖胶 

分 类 号：Q949.28[生物学—植物学] R931.6[医药卫生—生药学]