氧化苦参碱、儿茶素、表儿茶素对鼻咽癌HNE-1、HNE-1(200)细胞增殖抑制作用  

Effect of Oxymatrine,Catechin,Epicatechin on Inhibiting Cells Proliferation in HNE-1 and HNE-1(200) Cell Line

作　　者：王驰[1] 叶琳[2] 沈娜[2] 张潜英[2] 余晓燕[2] 陈鸿雁[2] 

机构地区：[1]重庆医科大学药学院,重庆400016 [2]重庆医科大学附属第一医院,重庆400016

出　　处：《肿瘤学杂志》2009年第7期619-621,共3页Journal of Chinese Oncology

基　　金：重庆市中医药局资助课题(2003-B-90)

摘　　要：[目的]研究氧化苦参碱、儿茶素、表儿茶素对鼻咽癌HNE-1及HNE-1(200)细胞的增殖抑制作用。[方法]以鼻咽癌HNE-1细胞株和放射照射诱导的HNE-1(200)耐药细胞株为研究对象,以维拉帕米为对照,采用MTT法测定氧化苦参碱、儿茶素、表儿茶素对两种细胞的增殖抑制率。[结果]氧化苦参碱对两种实验细胞均有抑制作用,且这种抑制作用呈浓度依赖性;氧化苦参碱的细胞抑制作用与维拉帕米相近。表儿茶素和儿茶素在本试验浓度下对两种细胞抑制作用不明显,抑制率均在20%以下。[结论]氧化苦参碱有明显的细胞抑制作用,表儿茶素和儿茶素未发现明显细胞毒作用。[Purpose ] To investigate the effects of oxymatrine, catechin and epicatechinon on inhibiting cell proliferation in HNE-1 and HNE-1 （200） cell line. [Methods] NPC HNE-1 cell line and muhidrug resistant HNE-1（200） subline were used in the study. Inhibiting proliferation rate of oxymatrine, catechin and epicatechinon in two cell lines was detected by MTT. [ Results ] Oxymatrine inhibited cell proliferation in both cell lines with a concentrative dependent effect, it was similar to Verapamil. Epicatechin and catechin had no distinct inhibiting effect in both cell lines with the same experiment concentration. [Conclusions] Oxymatrine has markedly inhibiting effect on HNE-1 and HNE-1 （200） cells growth, while Catechin and epicatechin do not have such inhibiting effect to these cell lines.

关 键 词：氧化苦参碱 儿茶素 表儿茶素 HNE-1 

分 类 号：R739.63[医药卫生—肿瘤]