Inhibitory effects of matrine on electrical signals and amino acid neurotransmitters in hippocampal brain slices  

作　　者：Xuping Wang Jiping Chen Guizhi Zhao Dan Shou Xuezhi Hong Jianmin Zhang 

机构地区：[1]Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, Zhejiang Province, China [2]Second Affiliated -Hospital of Zhejiang University School of Medicine, Hangzhou -310009, Zhejiang Province, China

出　　处：《Neural Regeneration Research》2009年第7期536-540,共5页中国神经再生研究（英文版）

摘　　要：BACKGROUND： Studies on electrical signals of hippocampal brain slices in vivo have shown that matrine inhibits benzylpenicillin sodium-induced activation of neuronal signal transduction. OBJECTIVE： To verify the inhibition effect of matrine on activation of electrical signals in rat brain slices and the role rnatrine plays in hippocampal amino acid transmitter release. DESIGN, TIME AND SETTING： The in vitro, neurophysiological, controlled experiment was performed in the Zhejiang Province Key Laboratory of Cardio-cerebrovascular Disease and Nerve System Drugs Appraisement and Chinese Traditional Medicine Screening and Research between July 2003 and May 2004. The in vivo, neuronal, biochemical experiment was performed in the Zhejiang Province Key Laboratory of Chinese Traditional Medicine Quality Standardization from July 2005 to December 2006. MATERIALS： Forty healthy, Sprague Dawley rats, 7-8 weeks old, and 120 healthy, ICR mice, 5-6 weeks old, were included in this study, irrespective of gender. Matrine powder was provided by the National Institute for the Control of Pharmaceutical and Biological Products, China. Matrine injection was purchased from Zhuhai Biochemical Pharmaceutical Factory, China. Penicillin was bought from Shijiazhuang Pharmaceutical Group Co., Ltd., China. METHODS： （1） Rats were randomly assigned to four groups： control, penicillin model, and matrine high-dose and low-dose, with 10 rats in each group. The control group was perfused with artificial cerebrospinal fluid. In the remaining three groups, hippocampal brain slices were perfused with normal artificial cerebrospinal fluid containing 1 × 106 U/L penicillin for the first 10 minutes. The penicillin model group received artificial cerebrospinal fluid for an additional 30 minutes, while the matrine high-dose and low-dose groups received 0.1 g/L and 0.05 g/L matrine, respectively, for an additional 30 minutes. （2） Mice were randomly assigned to four groups （n = 30）. The matrine high-, medium-, and low-dose groups werBACKGROUND： Studies on electrical signals of hippocampal brain slices in vivo have shown that matrine inhibits benzylpenicillin sodium-induced activation of neuronal signal transduction. OBJECTIVE： To verify the inhibition effect of matrine on activation of electrical signals in rat brain slices and the role rnatrine plays in hippocampal amino acid transmitter release. DESIGN, TIME AND SETTING： The in vitro, neurophysiological, controlled experiment was performed in the Zhejiang Province Key Laboratory of Cardio-cerebrovascular Disease and Nerve System Drugs Appraisement and Chinese Traditional Medicine Screening and Research between July 2003 and May 2004. The in vivo, neuronal, biochemical experiment was performed in the Zhejiang Province Key Laboratory of Chinese Traditional Medicine Quality Standardization from July 2005 to December 2006. MATERIALS： Forty healthy, Sprague Dawley rats, 7-8 weeks old, and 120 healthy, ICR mice, 5-6 weeks old, were included in this study, irrespective of gender. Matrine powder was provided by the National Institute for the Control of Pharmaceutical and Biological Products, China. Matrine injection was purchased from Zhuhai Biochemical Pharmaceutical Factory, China. Penicillin was bought from Shijiazhuang Pharmaceutical Group Co., Ltd., China. METHODS： （1） Rats were randomly assigned to four groups： control, penicillin model, and matrine high-dose and low-dose, with 10 rats in each group. The control group was perfused with artificial cerebrospinal fluid. In the remaining three groups, hippocampal brain slices were perfused with normal artificial cerebrospinal fluid containing 1 × 106 U/L penicillin for the first 10 minutes. The penicillin model group received artificial cerebrospinal fluid for an additional 30 minutes, while the matrine high-dose and low-dose groups received 0.1 g/L and 0.05 g/L matrine, respectively, for an additional 30 minutes. （2） Mice were randomly assigned to four groups （n = 30）. The matrine high-, medium-, and low-dose groups wer

关 键 词：MATRINE HIPPOCAMPUS evoked field potential amino acid 

分 类 号：Q42[生物学—神经生物学] S816.7[生物学—生理学]